CORRELATION OF BLOOD BIOCHEMICAL INDICATORS WITH THE LEVEL OF KNEE JOINT DAMAGE IN THE MODEL OF THE POSTTRAUMATIC OSTEOARTHRITIS.

The aim of the study was to assess the dependence of blood biochemical parameters with the degree of osteoarthritis of the knee joint and therapy. In experiments, osteoarthritis was simulated in rabbits (n=25) and after 75 days changes in the level of peroxidation products, activity of leukocyte enzymes were studied and the results were compared with the degree of knee joint injury, as well as taking into account the application of a concentrate of bone marrow aspirate, mechanically homogenized adipose tissue and platelet rich plasma. Analysis of the results confirmed the assumption of a direct relationship between the level of peroxidation products (diene conjugates, TBA-reactive products, products of oxidative modification of proteins), ceruloplasmin, and proinflammatory activity of leukocytes (by the activity of elastase and myeloperoxidase) with the level of morphological changes in the articular cartilage, capsule and meniscus of the knee joint.

[Cardioprotective action of coenzyme Q in conditions of its endogenous synthesis activation in cardiac ischemia-reperfusion in old rats].

The changes of functional state isolated by Lanhendorf old rat hearts with low content of ubiqinone--coenzyme Q (CoQ) under activation of it endogenous synthesis through administration of precursors--4-hydroxybenzoic acid, methionine and modulator vitamin E were studied. The activation of ubiqinone biosynthesis contribute to cardioprotective effect due to reduce the degree of the ischemia-reperfusion injury in old rat heart, namely the restoration of myocardial contractile function and coronary flow as well as decrease the end diastolic pressure and oxygen cost of the heart compared with control group of the animals during ischemia-reperfusion. Thus the results allow to conclude that the activation of KoQ biosynthesis under administration of it precursors has protective effect in the development of the heart postreperfusion damages in aging.

[In vivo effect of precursors of ubiquinone biosynthesis on sensitivity of mitochondrial permeability transition pore in the heart of old rats].

This research work is devoted to study of the sensitivity of mitochondrial permeability transition pore (MPTP) opening to its inductors--ions Ca2+ (10(-4) mol/l) and oxidant phenylarsine oxide (10(-4) mol/l) and content of ubiquinone (coenzyme Q, CoQ) and vitamin E in heart mitochondria of adult, old (control) and old rats under administration of precursors an modulator of CoQ biosynthesis--4-hydroxybenzoic acid, methionine and modulator of CoQ biosynthesis, namely vitamin E. The results of our research demonstrate that administration of complex of biologically active substances, which are precursors and modulators of CoQ biosynthesis, leads to decrease in the sensitivity of MPTP opening to its inductors and increase of CoQ and vitamin E content in old rats heart mitochondria. Therefore the results obtained lead to a conclusion that increase of CoQ content due to administration of precursors and modulator of its biosynthesis is an effective way in the inhibition of MPTP opening.

[Physiological aspects of ubiquinone supplementation in cardiovascular pathology].

Ubiquinone (coenzyme Q10) is an obligatory component of the respiratory chain in the inner mitochondrial membrane coupled to ATP synthesis and acts as an antioxidant. Its additional localization in the different subcellular fractions is probably associated with its multiple functions in the cell. Coenzyme Q10 deficiency has been observed in patients with congestive heart failure, angina pectoris, coronary artery disease, cardiomyopathy, hypertension, mitral valve prolapse.

[Biochemical properties and functional role of ubiquinone (CoQ). Aspects of practical use].

In addition to well known function of ubiquinone as a component of mitochondrial electron-transport chain and antioxidant, the latest research data that evidence for its function as a regulator of the processes connected with gene expression, signal transduction, including regulation of apoptosis are considered in this review. We also discuss practical aspects of use of ubiquinone for prophylaxis and treatment of cardiovascular, oncological diseases, pathologies of immune system, disorders caused by the effect of ionizing radiation.