Detailed protocol for germ-free colonization with spp. biofilms.

In this protocol, we describe a germ-free model to investigate anaerobic bacterial biofilms. We detail how to establish spp. biofilms in the fruit fly's gut using an easy to carry out method.

Extracellular DNase BmdE Targeting Biofilm Matrix, a Novel Inter-Species Competition Mechanism.

Acne vulgaris is the most common dermatological disorder worldwide affecting more than 80% of adolescents and young adults with a global prevalence of 231 million cases in 2019. The involvement of the skin microbiome disbalance in the pathophysiology of acne is recognized, especially regarding the relative abundance and diversity of a well-known dominant human skin commensal. Biofilms, where bacteria are embedded into a protective polymeric extracellular matrix, are the most prevalent life style for microorganisms.

In vivo model of Propionibacterium (Cutibacterium) spp. biofilm in Drosophila melanogaster.

Objectives: Acne vulgaris is a common inflammatory disorder of the pilosebaceous unit and Propionibacterium acnes biofilm-forming ability is believed to be a contributing factor to the disease development. In vivo models mimicking hair follicle environment are lacking. The aim of this study was to develop an in vivo Propionibacterium spp.