Influence of bacterial lectins on some reactions of nonspecific immunity in sarcoma 37 transplanted mice.

Aim: To study preventive effect of cytotoxic lectin from Bacillus subtilis B-7025 on the tumor growth and nonspecific immunity in sarcoma 37 transplanted mice.

Methods: Sarcoma 37 cells were transplanted in Balb/c mice. Cytotoxic lectin (CL) was isolated from cultural fluid of B.

Elevation of the efficacy of antitumor vaccine prepared on the base of lectines from B. subtilis B-7025 upon its combined application with probiotics in vivo.

Unlabelled: The aim of the work was to evaluate in vivo the possible enhancement of efficacy of antitumor vaccine prepared on the base of cytotoxic lectine from B. subtilis B-7025 by its combination with probiotic mixture of Enterococcus faecium K-50 and Saccharomyces cerevisiae 14K or their metabolic products.

Materials And Methods: Experiments were carried out on the models of solid sarcoma 37 (S37) and metastatic Lewis lung carcinoma (3LL).

[Lectin activity of antitumor substances synthesized by Bacillus subtilis B-7025].

Nutrient medium, which is optimal for synthesis of biologically active substances and allows obtaining stable outflow of Bacillus subtilis B-7025 to the culture medium, has been selected under the conditions of the bacillus periodic cultivation. It has been established that these substances are extracellular lectins with high activity and carbohydrate specificity to fructose-1.6-di phosphate, N-acetylneuraminic acid and glucose derivatives: D-glucuronic acid and D-glucosamine.