[Germline and Somatic Mutations in Archived Breast Cancer Specimens of Different Subtypes].

Molecular profiling of tumors may provide promising options for personalized treatment. We have examined the spectrum of germline and somatic mutations in 23 breast cancers (ВС) of various molecular subtypes, including tumors 1) with expression of estrogen, progesterone and/or epidermal growth factor receptor HER2/neu, and 2) with a triple negative phenotype. Genomic DNA specimens were isolated from archived tumor and normal tissue samples and subjected to targeted sequencing of the coding regions of 25 cancer-associated genes with a mean coverage of x 1000.

[Morphometric and immunohistochemical markers of breast cancer progression].

The results of the study for the last decade aimed at the search of markers of mammary carcinoma malignancy and prognosis are reported. Accumulation and integration of various data contributes not only to more precise identification of the malignancy but also to more complete understanding of biological essence of mammary carcinoma.