[Parameters of hemolysis by sodium dodecyl sulfate as an indicator of the structural states of erythrocyte membranes].

The relationship between the parameters (percentage and rate) of hemolysis by sodium dodecyl sulfate and the time of incubation of human erythrocyte suspensions in glucose-free medium at 37 degrees C was studied. The polyphasic changes in the parameters were found, which depend on the mode of pretreatment: ATP depletion by iodoacetate, heat denaturation of spectrin, and treatment of cells by valinomycin. It was found that the percentage and rate of detergent hemolysis do not always change in parallel.

[Heterogeneity of pores formed in erythrocyte membrane by lipophilic hemolysins].

Concentration dependences of the rate of hemolysis are studied for ascorbic acid, sodium dodecylsulfate, triton X-100, ethyl and butyl alcohols. The double logarithmic plot of this dependence for ascorbic acid presents the straight line with the slope (n) of 2, meaning that two hemolytic molecules are necessary for the formation of one pore in the membrane. Other hemolysins are characterized by the curves with n changing from 2 to 24 as hemolysin concentration increases.