DNA methylation patterns of metaphase chromosomes in human preimplantation embryos.

We performed a stage-by-stage study of DNA methylation patterns in metaphase chromosomes from blastomeres of triploid and abnormal diploid human embryos. QFH-banded homologous parental chromosomes differ in their DNA methylation patterns at the metaphase of the 1st cleavage division. Chromosomes of both parental genomes are gradually demethylated at subsequent cleavages, undergoing hemimethylation in 2-cell embryos.

[Novel human embryonic stem cell lines C612 and C910].

Novel human embryonal stem cell lines C612 and C910 have been established from hatching blastocytes. Cells were cultivated in mTeST medium on mouse fibroblast feeder-layers. They express common pluripotent markers such as alkaline phosphatase, Oct 3/4, SEEA-4, Nanog, Rex1.

[Morphological peculiarities of the adipose tissue and their significance for the ultrasound lipodestruction].

Using the autopsy material obtained from 20 human corpses, the samples of subcutaneous adipose tissue of the gluteal, abdominal, inguino-ileal and axillar areas, as well as the samples of mesenterial, retroperitoneal, mediastinal, pararectal and paranephral adipose tissue was studied histologically. The average sizes of the lobes and of adipocytes were measured, and the degree of the development of connective tissue elements was determined. Adipose tissue was found to have a similar general structural plan in all the areas studied, however the differences were noted in the thickness of the interlobular connective tissue septae and in tissue vascularization.

[High-intensity ultrasonic destruction of fatty tissue in the course of lymphatic dissection in oncological patients].

The paper discusses experimental findings in support of ultrasound destruction of fat tissue at different sites. Rate of destruction was designed to match that of development of the connective-tissue stroma of adipose tissue and size of adipose cells. Parameters of adipose tissue framework at different sites may be taken into account during extended lymph node dissection using ultrasound liposuction in oncological practice.

[Evaluation of the state of the gene pool of natural populations of vertebrate animals inhabiting the fragmented landscape of Moscow and Moscow district (with special reference to brown frogs)].

A system of urban ecological genetic monitoring has been developed for the first time in Russia and in the world. As a model, two species of brown frogs, Rana arvalis Nilss. and R.