[Investigation of peroxide modification of low- and very low-density lipoproteins in patients with coronary heart disease by the method of fluorescent spectroscopy].

In the total fraction of low and very low density lipoproteins (LDL+VLDL) isolated from serum by precipitation in the presence of heparin-Mn the copper-induced lipid peroxidation was accompanied by accumulation of LPO products, a decrease ANS fluorescence intensity (F(ANS)) and an increase in probe--cation DSP-6, a fluorescence intensity decrease of intrinsic in the ultraviolet area (F(uv)) and an increase in the visible area (F(vis)). The degree of lipoprotein modification was estimated by calculating the F(vis)/F(uv) and F(DSP)-6/F(ANS) ratio. Strong positive correlation was found between these ratios and concentration of thiobarbituric acid-reactive substances (TBARS) of LDL+VLDL samples isolated from sera of 49 donors and incubated at 37 degrees C in the presence of 50 M CuSO4 during 0, 3 and 24 hr (F(vis)/F(uv) (r = 0.

[Study of blood cholesterol and triglycerides in patients with acute myocardial infarction].

The levels of cholesterol (C) and triglycerides (TG) were studied by a standard biochemical assays and the amount of C and TG was examined by a fluorescence assay in 55 patients with complicated (n=32) and uncomplicated (n=23) acute myocardial infarction (AMI) and in 25 apparently healthy donors. The content of C did not differ significantly in the study groups. As compared with the controls, the patients with complicated AMI had lower values of C + TG (p < 0.