[Genome-wide lentivector-based pooled shRNA library optimization].

We have optimized lentiviral vector constructs and cassettes for expression of short hairpin RNAs (shRNAs) in order to create genome-wide library capable of inhibition of full variety of human mRNAs. The vector optimization has resulted in 15-20-fold improvement in virus stock titers. We found that in the context of lentiviral vector the most effective structure for the shRNA is simple hairpin with 21 nucleotide stem.

[Down-regulation of TRIP6 expression induces actin cytoskeleton rearrangements in human carcinoma cell lines].

The structure and dynamics of actin cytoskeleton are factors important for regulation of cell adhesion, spreading, and migration. TRIP6 is a LIM domain-containing protein interacting with many actin cytoskeleton- associated proteins and modulating the activity of certain transcription factors. To study the functions of TRIP6, we inhibited its expression in A549 and A431 cells with short interfering RNAs (siRNAs).