[Prevention of antibiotic-associated diarrhea in children].

One of the most frequent complications of antibiotic therapy is a diarrheal syndrome, which may be related both to the direct toxic effects of antibiotics on the gastrointestinal tract, and the activation of conditionally pathogenic intestinal flora. Particular attention gets to present the problem of C. difficile-infection, which may be the consequence of the development of pseudomembranous colitis in both children and adults.

[Cultivation of cells on a surface covered by microspheres with coupled histones].

The use of histones for modification of the surface intended for cultivation of cells was studied. The work was carried out on the cell line 293 of human embryonic kidney transformed by adenovirus (Ad5) and on the cell line BALB/3T3 clone A31 of mouse spontaneous transformed embryonic fibroblasts. We analyzed interaction of cells with histones of different types put on a hydrophobic surface or on dextran microspheres with diameters of 1.

[Production and some properties of covalent conjugates of total histone and kanamycin].

A complex containing total histone and antibiotic kanamycin was produced under optimal conditions using glutaric aldehyde. The obtained water-soluble covalently bound conjugates of these substances were identified by means of enzyme labelled immunoassay and their molecular mass estimated. The validity of new class of highly effective drugs, which may be produced basing on the principles studied, was substantiated considering that histones exhibit the transport properties and are able to augment hematoencephalic barrier permeability.