[Conformational states of NADH molecules in hydrated shell under weak electromagnetic irradiation].

The work deals with spectra of capillary waves of water distillate and water solution of NADH and dynamics of NADH under electromagnetic irradiation in the range of millimeters at different space frequencies of surface wave k.

[The evaluation of effectiveness of application of automated system of polymerase chain reaction to detect meticillin-resistant and meticillin-sensitive Staphylococcus aureus in clinical material from trauma orthopedic patients].

The article considers the evaluation of effectiveness of application of automated polymerase chain reaction system GeneXpert DX ("Cepheid USA) as compared with bacteriologic method in detection of S. aureus (SA) and meticillin-resistant S. aureus (MRSA) under infection of skin and soft tissues in orthopedic patients.

[Estimation of species diversity of coagulase-negative staphylococci isolated in hospitals of Russian Federation in 2009-2010].

Aim: Comparative analysis of species diversity of sample of coagulase-negative staphylococci (CNS) isolated in hospitals of different specializations.

Materials And Methods: For identification of 102 CNS strains, biochemical systems manufactured by NPO "Diagnostic Systems", VITEK 2 Compact, and BBL Crystal as well as sequencing of fragments of tuf and gap genes were used.

Results: Greater differentiating capability of genotyping compared with phenotyping methods for species identification of staphylococci was demonstrated.

[Isolation rate of enterotoxigenic staphylococci in patients with sepsis, pneumonia and burns].

The occurrence of Staphylococcus aureus strains producing enterotoxins of types SEA and SEB, which isolated from patients of different profile and caused the infectious process accompanied by pronounced intoxication without vomiting and enteric disturbances, was determined by means of the indirect hemagglutination test. The collection included 28 strains isolated in sepsis, 38 strains isolated in pneumonia, 57 strains isolated from patients with burns and 23, from the hands and nasopharynx of the medical staff. Among the staphylococcal strains isolated in sepsis, 75.

[Study on the polymorphism of the coagulase gene by the method of sequencing in methicillin-resistant Staphylococcus aureus strains, isolated in hospitals in different regions of Russia and Belarus].

This method was used for typing of 31 Staphylococcus aureus methicillin-resistant (MRSA) strains; of these, 27 were clinical isolates obtained in hospitals of different cities of Russia and Belarus and 4 were international epidemic strains EMRSA-1, -2, -3, -12. The sequencing of the variable area, located in the middle part of the coagulase gene between nucleotides 979-1355 and detected with the use of information technologies, was carried out. The results of this sequencing were compared with those of the earlier study on the polymorphism of the area of the same gene between nucleotides 1513-2188, carried out by the method of PCR-restrictive fragment length polymorphism.