Changes in the Microbiome of Milk in Cows with Mastitis.

Analysis of milk micrbiomes from healthy cows and cows with different (clinical and subclinical) forms of mastitis was performed at two farms of the Central Russia. An increase in the operational taxonomic units (OTUs) of bacteria of the phylum Proteоbacteria belonging primarily to Pseudomonadales, Burkholderiales, as well as Streptococcaceae, Staphylococcaceae, and Bacillaceae in the animals with mastitis was detected. The Planococcaceae OTU percentage decreased.

Virulence Factors and Phylogeny of Associated With Bovine Mastitis in Russia Based on Genome Sequences.

is a causative agent of different infectious processes, food poisoning, and autoimmune disorders. The horizontal transfer of pathogenic strains can occur from animal to human under both house and farm conditions, and the spread of strains with antibiotic resistance is an existing problem. In addition to the spread of antibiotic-resistant strains in clinics, this problem also exists in veterinary medicine.

Draft Genome Sequences of Five Staphylococcus aureus Strains Isolated from Clinically Healthy Cows in the Russian Federation.

We present here the draft genome sequences of five strains isolated from milk samples from clinically healthy cows in the Russian Federation. Four of them were determined to be sequence type 97 (ST-97), and one was determined to be ST-22. All the strains are characterized by their genome possessing genes that code for enterotoxins and cytotoxins.

Exotoxin diversity of Staphylococcus aureus isolated from milk of cows with subclinical mastitis in Central Russia.

Mastitis, a major veterinary problem widespread in many regions, is caused mainly by Staphylococcus spp. However, there is no current reliable information about the role of Staphylococcus aureus and their toxins in the development of mastitis in cows in the territory of the Russian Federation. The aim of this investigation was to determine the profile of exotoxins of S.

Association of Polymorphisms in Innate Immunity Genes TLR9 and DEFB1 with Human Longevity.

The distribution of polymorphic variants of innate immunity genes TLR9 (+2848G>A) and DEFB1(-20G>A; -44C>G; -52G>A) was evaluated in long-living individuals. No significant differences were found in the distribution of genotypes and alleles of the TLR9 gene. The following features were revealed: increase in the frequency of AA and GG genotypes and decrease in the frequency of the AG genotype of the DEFB1(-20G>A) gene; increase in the frequency of the CC genotype and C allele and decrease in the frequency of CG and GG genotypes and G allele of the DEFB1(44C>G) gene; and increase in the frequency of AA and AG genotypes and A allele and decrease in the frequency of the GG genotypes and G allele of the DEFB1(-52G>A) gene.