J Coll Physicians Surg Pak
September 2017
We report a case of secondary cold agglutinin disease (CAD) due to pulmonary tuberculosis in a 68-year male patient who presented with acrocyanosis involving both upper limbs and greater portion of lower limbs. Direct Coombs' test was positive with mild anemia and the cold agglutinin titer was high. Sputum showed numerous acid fast bacilli per high power field.
View Article and Find Full Text PDFBackground: The prevalence of chronic Hepatitis B Virus (HBV) infection is 2-4% in the Pakistani population, defining Pakistan as an intermediate prevalence country. In this study, hepatitis B surface antigen (HBsAg) reactive blood donations were screened using a combination of serological and molecular methods to identify immune escape HBV mutant strains and to determine the HBV genotypes and subtypes present in Pakistan.
Methods: Blood donations were collected at the Armed Forces Institute of Transfusion (AFIT) located in northern Pakistan and the Hussaini Blood Bank (HBB) located in the south.
Objective: To determine the seroprevalence of Human T-cell Lymphotropic Virus-1/2 (HTLV-1/2) in blood donors in Northern Pakistan.
Study Design: Descriptive study.
Place And Duration Of Study: Armed Forces Institute of Transfusion, Rawalpindi, from July to August 2013.
Background: The Armed Forces Institute of Transfusion located in Rawalpindi, Northern Pakistan, acts as a regional blood center with more than 50,000 donations collected annually. Nucleic acid amplification testing (NAT) was introduced in our institution in September 2012 for screening all seronegative blood donors.
Study Design And Methods: The study was conducted from September 21, 2012, to September 20, 2013.
Transfus Apher Sci
August 2014
Objective: Find the allele frequencies of Duffy blood group antigens in donor population from northern Pakistan.
Design: Cross sectional study.
Place And Duration Of Study: Armed Forces Institute of Transfusion (AFIT), Rawalpindi in year 2012.
Background: Deferring blood donors who admit to high-risk behavior on questioning are likely to eliminate those in window period for transfusion transmitted infections (TTI). However, many questions have been implemented in some countries as part of donor history questionnaire, based on precautionary principle and not on evidence, and can result in increased donor losses. This study aims to identify effective risk-directed questions having high predictive value, in local context which can form part of blood donor deferral policies.
View Article and Find Full Text PDFJ Ayub Med Coll Abbottabad
June 2008
Background: Safety of blood donors rest on withdrawing only appropriate quantities of blood. Adjusting the volumes drawn according to the average blood volumes of any population can ensure this. This requires knowledge of total blood volume of donors, which should ideally be measured by standard methods or derived by alternate suitable method.
View Article and Find Full Text PDFBackground: The purpose of this study was to determine the seroprevalence of anti-hepatitis B core antigen (HBc) and the impact of its testing along with other markers of hepatitis B, hepatitis B virus (HBV) DNA, hepatitis C virus antibody (anti-HCV), and syphilis in Pakistani blood donors.
Study Design And Methods: The study design was cross-sectional. A total of 966 donors were selected randomly for testing of anti-HBc and HBV markers, including HBV DNA, of 94,177 blood donors who were routinely screened for hepatitis B surface antigen (HBsAg), anti-HCV, human immunodeficiency virus antibody (anti-HIV), Treponema pallidum hemagglutination assay (TPHA), and malarial parasites from 2003 to October 2005.
A middle-aged lady presented with fever and splenomegaly and had been provisionally treated for malaria, typhoid and tuberculosis. Diagnostic splenectomy was performed which revealed diffuse large cell lymphoma, B type, localized to spleen. Patient had remission of disease after splenectomy.
View Article and Find Full Text PDFObjective: To prepare good quality screening cells reagent according to the standards, at Armed Forces Institute of Transfusion (AFIT).
Methods: Random group O donors, seronegative for HBsAg, HCV and HIV were selected if they resided in Rawalpindi or Islamabad and could be contacted. Micro column Gel technique was used to find out R1R1, R1wr, R2R2 and rr phenotypes with or without K antigen.
Objective: To find out the frequency, pattern and factors influencing red cell immunization secondary to multiple blood transfusions in patients of beta-thalassaemia major.
Design: A cross-sectional study.
Place And Duration Of Study: Armed Forces Institute of Transfusion, Rawalpindi, in November 2002.