Rapid detection of and vancomycin resistance using recombinase polymerase amplification.

Vancomycin-resistant enterococci (VRE), especially , have been a global concern, often causing serious healthcare-associated infections. We established a rapid approach for detecting and vancomycin-resistance genes ( and ) in clinical samples using isothermal recombinase polymerase amplification (RPA) combined with a lateral-flow (LF) strip. Specific RPA primer sets and probes for (to identify the presence of ) and genes were designed.