Publications by authors named "Nurupa Ramkissoon"
Article Synopsis
- An obstetric fistula is a severe complication from childbirth that creates an abnormal connection between the urinary tract or gastrointestinal tract and the genital tract, often due to prolonged and obstructed labor.
- Contributing factors like early marriage, poverty, and lack of access to proper healthcare have led to a high incidence of this condition in Ethiopia.
- Australian doctors Reginald and Catherine Hamlin moved to Ethiopia in 1959, developed a surgical technique for fistula repair, and founded the Addis Ababa Fistula Repair Hospital, helping over 60,000 women with transformative surgeries.
CRISPR/Cas-based gene-editing technologies have emerged as one of the most transformative tools in genome science over the past decade, providing unprecedented possibilities for both fundamental and translational research. Following the initial wave of innovations for gene knock-out, epigenetic/RNA modulation, and nickase-mediated base-editing, recent efforts have pivoted towards long-sequence gene editing- specifically, the insertion of large fragments (>1 kb) into the endogenous genome. In this review, we survey the development of these CRISPR/Cas-based sequence insertion methodologies in conjunction with the emergence of novel families of editing enzymes, such as transposases, single-stranded DNA-annealing proteins, recombinases, and integrases.
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