Enabling growth-decoupled recombinant protein production based on the methanol-free P promoter.

The current transition towards the circular bioeconomy requires a rational development of biorefineries to sustainably fulfill the present demands. The use of () can meet this challenge, since it has the capability to use crude glycerol as a carbon-source, a by-product from the biodiesel industry, while producing high- and low-added value products. Recombinant protein production (RPP) using has often been driven either by the methanol induced promoter (P ) and/or the constitutive promoter (P ).