Scrutinising the relationship between major physiological and compositional changes during 'Merrill O'Henry' peach growth with brown rot susceptibility.

In the present work, the major physiological and compositional changes occurring during 'Merrill O'Henry' peach growth and its relationship with susceptibility to three strains of spp. at 49, 77, 126 and 160 days after full bloom were explored. Results of disease incidence indicated wide differences among phenological stages, being 49 and 126 days after full bloom the moment when peaches showed significantly lower susceptibility to brown rot (40 and 23% of rotten fruit, respectively, for strain ML8L).

Labeling of with GFP and Its Validation for Studies on Host-Pathogen Interactions in Stone and Pome Fruit.

To compare the infection process of on nectarines and apples using confocal microscopy it is necessary to transform a pathogenic strain with a construct expressing a fluorescent chromophore such as GFP. Thus, germinated conidia of the pathogen were transformed with carrying the plasmid pPK2-hphgfp that allowed the expression of a fluorescent Hph-GFP chimera. The transformants were selected according to their resistance to hygromycin B, provided by the constitutive expression of the - gene driven by the glyceraldehyde 3P dehydrogenase promoter of .

Double-sided battle: The role of ethylene during Monilinia spp. infection in peach at different phenological stages.

Controversy exists on whether ethylene is involved in determining fruit resistance or susceptibility against biotic stress. In this work, the hypothesis that ethylene biosynthesis in peaches at different phenological stages may be modulated by Monilinia spp. was tested.

Exploring sources of resistance to brown rot in an interspecific almond × peach population.

Background: Monilinia spp. are responsible for brown rot, one of the most significant stone fruit diseases. Planting resistant cultivars seems a promising alternative, although most commercial cultivars are susceptible to brown rot.