Combinatorial treatment with β-glucanase enzyme and chlorhexidine induces cysticidal effects in Acanthamoeba cyst.

Acanthamoeba cysts have a cellulose cell wall made up of a solid layer of β-glucan, which confers resistance to the dormant phase of this microorganism. The ability of Acanthamoeba to change to this dormant phase causes difficulties in treating its infection at the cyst stage as compared to the trophozoite stage. Therefore, targeting cyst total mortality can help to prevent re-infection in patients.

trehalase enzyme induced morphological and protein alterations on cyst and molecular docking studies.

The cytotoxicity of is yet to fully illustrate due to recalcitrant of during cyst stage. The formation of the trehalose layer at the cyst stage protects the inner components of this opportunist protozoan parasite. Trehalase from the (AnTre) activity on the cyst of was determined based on AnTre dose-response, morphological and protein changes.