Second internal transcribed spacer (ITS-2) as genetic marker for molecular characterization of in rabbits from several areas of East Java, Indonesia.

Objectives: The purpose of this study is to use the second internal transcribed spacer (ITS-2) to determine the molecular characteristics of in rabbits from several areas of East Java.

Methods: Collecting mites from rabbits with clinical signs of scabies; DNA extraction with minikit QIAamp DNA; polymerase chain reaction amplification; nucleotide sequence analysis; homology and phylogenetic tree using the Neighbor-Joining method in the program molecular evolutionary genetics analysis-7 (MEGA-7).

Results: Sequence analysis of ITS-2 from five regions in East Java showed an identity >91.