Development of a Spectrophotometric Assay for the Cysteine Desulfurase from .

: Antibiotic-resistant represents a growing threat in the modern world, and new antibiotic targets are needed for its successful treatment. One such potential target is the pyridoxal-5'-phosphate (PLP)-dependent cysteine desulfurase (SufS) of the SUF-like iron-sulfur (Fe-S) cluster biogenesis pathway upon which relies exclusively for Fe-S synthesis. The current methods for measuring the activity of this protein have allowed for its recent characterization, but they are hampered by their use of chemical reagents which require long incubation times and may cause undesired side reactions.