Publications by authors named "Nunta Churngchow"

Article Synopsis
  • Phytophthora palmivora is a plant pathogen linked to significant crop damage, particularly in papaya, yet the mechanisms behind its infection are not fully understood.
  • Researchers identified a secreted glycoprotein called Ppal15kDa which, when manipulated in genetic experiments, significantly influenced the pathogen's ability to infect host plants.
  • The study suggests that Ppal15kDa is crucial for both the development of infection structures and overall pathogenicity in P. palmivora, highlighting its potential role in similar pathogens.
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Elicitors play an important role in plant and pathogen interactions. The discovery of new elicitors and their effects on plant defense responses is significant and challenging. In this study, we investigated novel elicitors from and their effects on plant defenses.

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The brown seaweed ( C. Agardh-Sargassaceae) extract was examined for its bioelicitor properties in the rubber tree seedling ( (Willd. ex A.

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Elicitors from seaweeds are considered an alternative stimulant of plant defenses against pathogenic infection. Finding new sources of elicitors and exploring their effects on plant defenses is a significant undertaking. In this study, we extracted crude polysaccharide (CPS) from (a red alga) and tested the effects of the compound on rubber tree () defense responses.

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Induced resistance by elicitors is considered to be an eco-friendly strategy to stimulate plant defense against pathogen attack. In this study, we elucidated the effect of salicylic acid (SA) on induced resistance in rubber tree against and evaluated the possible defense mechanisms that were involved. For SA pretreatment, rubber tree exhibited a significant reduction in disease severity by 41%.

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Rubber tree (Hevea brasiliensis Muell. Arg) is an important economic crop in Thailand. Leaf fall and black stripe diseases caused by the aggressive oomycete pathogen Phytophthora palmivora, cause deleterious damage on rubber tree growth leading to decrease of latex production.

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Article Synopsis
  • This report introduces HbPR-1, a cDNA from rubber tree (Hevea brasiliensis) that may help combat the pathogen Phytophthora palmivora after treatment with salicylic acid.
  • The HbPR-1 protein, characterized as a basic PR-1 type, was expressed in Nicotiana benthamiana, showing its potential to enhance resistance against the pathogen by reducing necrosis areas.
  • Additionally, the purified recombinant HbPR-1 protein demonstrated a 64% inhibition of P. palmivora zoospore germination, indicating its effectiveness as an antimicrobial agent.
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A novel cDNA encoding a bi-functional α-amylase/subtilisin inhibitor (HbASI) was isolated from rubber (Hevea brasiliensis) leaves cultivar RRIM600. The HbASI had strong homology with the soybean trypsin inhibitor (Kunitz) family of protease inhibitors. Its putative amino acid sequence was similar to that of the α-amylase/subtilisin inhibitor from Ricinus communis (72% identity).

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Peroxidases are oxidoreductase enzymes produced by most organisms. In this study, a peroxidase was purified from Hevea brasiliensis cell suspension by using anion exchange chromatography (DEAE-Sepharose), affinity chromatography (Con A-agarose) and preparative SDS-PAGE. The obtained enzyme appeared as a single band on SDS-PAGE with molecular mass of 70 kDa.

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Polyphenol oxidase (PPO) was examined from the extract of leaf, seed, and cell suspension of Hevea brasiliensis, a rubber plant. The defense-related isozyme from Hevea cell suspension induced by culture filtrate of Phytophthora palmivora or by agitation stress was isolated through anion exchange and affinity chromatography, respectively. A 104-purification fold, migrated as a single band of 70 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis of PPO, was obtained after further purified by the preparative gel electrophoresis.

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