Rapid alignment-free bacteria identification via optical scattering with LEDs and YOLOv8.

Rapid and accurate bacterial identification is essential for timely treatment of infections like sepsis. While traditional methods are reliable, they lack speed, and advanced molecular techniques often suffer from cost and complexity. The bacterial detection technology based on optical scattering system offers a rapid, label-free alternative but traditionally relies on complex lasers and analysis.

A novel portable Raman scattering platform for antibiotic screening in pig urine.

Background And Aim: Public health and food safety are gaining attention globally. Consumer health can be protected from chemical residues in meat by early detection or screening for antibiotic residues before selling the meat commercially. However, conventional practices are normally applied after slaughtering, which leads to massive business losses.

Microbial Poly(hydroxybutyrate-co-hydroxyvalerate) Scaffold for Periodontal Tissue Engineering.

In this study, we fabricated three dimensional (3D) porous scaffolds of poly(hydroxybutyrate-co-hydroxyvalerate) with 50% HV content. P(HB-50HV) was biosynthesized from bacteria H16 and the in vitro proliferation of dental cells for tissue engineering application was evaluated. Comparisons were made with scaffolds prepared by poly(hydroxybutyrate) (PHB), poly(hydroxybutyrate-co-12%hydroxyvalerate) (P(HB-12HV)), and polycaprolactone (PCL).

Organelle specific simultaneous Raman/green fluorescence protein microspectroscopy for living cell physicochemical studies.

We demonstrate a novel bio-spectroscopic technique, "simultaneous Raman/GFP microspectroscopy". It enables organelle specific Raman microspectroscopy of living cells. Fission yeast, Schizosaccharomyces pombe, whose mitochondria are green fluorescence protein (GFP) labeled, is used as a test model system.

Detection of Metal-Molecule-Metal Junction Formation by Surface Enhanced Raman Spectroscopy.

Vibrational modes play a key role in characterizing metal-molecule-metal junctions, but their detection currently either requires single-molecule sensitivity or the generation of defect-free large-scale junctions. Here we demonstrate that surface-enhanced Raman scattering (SERS) on nonideal surfaces can provide a significant amount of information despite many defects in the layer. We determine the vibrational signature of the molecular electronic junction for palladium ions complexed and reduced on 4-mercaptopyridine adsorbed on rough gold and gold nanoparticles using SERS and density functional theory.

Discrimination between hydrogen bonding and protonation in the spectra of a surface-enhanced Raman sensor.

We investigate the surface-enhanced Raman spectra of 4-mercaptopyridine on gold in a variety of acids. 4-Mercaptopyridine is a known pH sensor which exhibits characteristic spectral changes when the pH is changed. Here we show with the help of experiment and density functional calculations that the ring breathing mode is also highly sensitive to hydrogen bonding.