Achieving synchronous nitrogen and phosphorus removal by aerobic enrichment of electrotrophic/heterotrophic bacteria and denitrifying polyphosphate-accumulating organisms in repeatedly oxygen-rich microbial fuel cells.

Realizing the enrichment of functional bacteria in microbial fuel cells (MFCs) for wastewater treatment holds substantial research significance. This study explored a novel method of repeatedly oxygen-rich anode environment to enrich electrotrophic/heterotrophic bacteria (EHB) and denitrifying polyphosphate-accumulating organisms (DPAOs) in membrane-less single-chamber air-cathode (AC) MFCs to treat household wastewater. Repeated accumulation of higher dissolved oxygen (DO) was conducive to enhancing the growth of EHB and DPAOs.

Revealing the performance of aerotolerant anodes for electroactive nitrification/denitrification and current production under coexistence of oxygen and nitrate conditions.

The coexistence of oxygen and/or nitrate at anode usually affects the biofilm activities of traditional anaerobic anode, thereby deteriorating wastewater treatment performance of microbial fuel cells (MFCs). Improving the aerotolerant responses of anode biofilms is a challenge for field application. In this study, we report that using the electroactive nitrifying/denitrifying inoculum and air-cathode expansion could fabricate the aerotolerant anode biofilms (AAB) under affordable nitrate stress (90 ± 5 mg/L).

Impact of soil fissure status on microbial community in mining-disturbed area, the northern Shaanxi province.

Mining disturbance has great impacts on soil physicochemical factors, causing notable differences between pre-mining and after-mining conditions, and between coal mining areas and non-mined areas. However, little is known about whether the fissure statuses induced by mining activities affect the edaphic factors and how soil microbial communities respond to these fissure development states. In this study, we systematically investigated the edaphic factors and microbial communities in a mining disturbance area exhibiting the full development status of soil fissures, where the sampling sites were divided into soil fissure development and closure zones.

Triple isotopes (δD, δO, δO) characteristic of river water and groundwater in an arid watershed from Qaidam Basin, Northwestern China: implications for hydrological cycle.

Combining traditional stable isotopes (δD and δO) and triple oxygen isotope (δO) is conducive to tracing hydrological cycle processes. The application of triple oxygen isotopes primarily focuses on precipitation, which is lacking in river water and groundwater. In this study, the spatial variations of δD, δO, δO, d-excess and O-excess of river water and groundwater in the Golmud River basin as well as the correlation between them were investigated to elucidate water origin and assess the evaporation influence on water bodies during flood season.

Large-scale surface water-groundwater origins and connectivity in the Ordos Basin, China: Insight from hydrogen and oxygen isotopes.

The sustainability of water resources is a major challenge for the Ordos Basin and Loess Plateau of China. The basis of effective water management is an understanding of the water cycle process. This study investigated the surface water-groundwater origins and connectivity using stable isotopes (δD and δO) of surface water and groundwater in 11 river basins in the Ordos Basin.

Deciphering three-dimensional bioanode configuration for augmenting power generation and nitrogen removal in air-cathode microbial fuel cells.

In this study, the engineering-oriented three-dimensional (3D) bioanode concept was applied, demonstrating that spiral-stairs-like/rolled carbon felt (SCF/RCF) configurations achieved good performances in air-cathode microbial fuel cells (ACMFCs). With the 3D anodes, ACMFCs generated significantly higher power densities of 1535 mW/m (SCF) and 1800 mW/m (RCF), compared with that of a traditional flat carbon felt anode (FCF, 315 mW/m). The coulombic efficiency of 15.

Insights into moisture sources and evolution from groundwater isotopes (H, O, and C) in Northeastern Qaidam Basin, Northeast Tibetan Plateau, China.

Knowledge of moisture sources is of great significance for the understanding of groundwater recharge and hydrological cycle. However, it is often difficult to identify the moisture sources and evolution especially in the areas with complex climate system. Isotopes in groundwater that acts as a climate archive provide a unique perspective on the moisture sources and evolution.

Coupling mixotrophic denitrification and electroactive anodic nitrification by nitrate addition for promoting current generation and nitrogen removal.

Nitrate promotes anodic denitrification and fasts organic matter removal in microbial fuel cells (MFCs). However, it suffers from poor total nitrogen (TN) removal and current recovery. In this study, some novel electroactive nitrifying/denitrifying bacteria (ENDB) were introduced in a single chambered air-cathode MFC to investigate the performance of this device and the microbial community shift by adding nitrate.

Microbial electrochemical driven anaerobic ammonium oxidation coupling to denitrification in a single-chamber stainless steel reactor for simultaneous nitrogen and carbon removal.

Anodic ammonium oxidation mainly focuses on autotrophic process, and the removal combined with organic matter oxidation is still unclear in microbial electrolysis cell (MEC). Here, a stainless-steel tank is constructed as an MEC for anaerobic ammonium oxidation and organic matter removal. Results show that MEC increases ammonium oxidation from 3.

A novel electrochemical sensor based on autotropic and heterotrophic nitrifying biofilm for trichloroacetic acid toxicity monitoring.

Trichloroacetic acid (TCA), a toxic substance produced in the disinfection process of wastewater treatment plants, will accumulate in the receiving water. The detection of TCA in the water can achieve the purpose of early warning. However, currently there are few reports on microbial sensors used for TCA detection, and the characteristics of their microbial communities are still unclear.

Membrane penetration of nitrogen and its effects on nitrogen removal in dual-chambered microbial fuel cells.

Owing to membrane penetration, a novel route of nitrogen removal was proposed in a dual-chamber microbial fuel cell with a proton exchange membrane (PEM). The results showed that NH-N rapidly migrated across PEM with a mass transfer coefficient (K) of 1.79 ± 0.

Aerobic biocathodes with potential regulation for ammonia oxidation with concomitant cathodic oxygen reduction and their microbial communities.

Aerobic biocathodes are effective construct for the simultaneous nitrification and denitrification, but the disturbance of cathodic oxygen reduction on ammonia oxidation and denitrification remains unclear. In this study, we revealed the oxygen reduction peak at -0.4 V (versus silver/silver chloride) by cyclic voltammetry analysis at a cathode without a biofilm.

Case Report: Clinical Features of a Chinese Boy With Epileptic Seizures and Intellectual Disabilities Who Carries a Truncated Variant.

The mental retardation-55 with seizures (MRD55) is a rare genetic disease characterized by developmental delay, intellectual disability, language delay and multiple types of epileptic seizures. It is caused by pathogenic variants of the gene, which encodes Nogo-B receptor (NgBR), a necessary subunit for the glycosylation reactions in mammals. To date, 25 disease-causing mutations of have been reported, which are responsible for various diseases, including dystonia, Parkinson's disease, developmental and epileptic encephalopathy as well as congenital disorder of glycosylation.

Electrode-dependent ammonium oxidation with different low C/N ratios in single-chambered microbial electrolysis cells.

Alternative method should be found to solve the ammonia accumulation in anaerobic digestion. Herein, electrode-dependent ammonium oxidation was successfully achieved in anaerobic single-chambered microbial electrolysis cells (MECs)under different low C/N ratios (0, 1, and 1.5), with an applied voltage of 0.

Integrated simultaneous nitrification/denitrification and comammox consortia as efficient biocatalysts enhance treatment of domestic wastewater in different up-flow bioelectrochemical reactors.

Simultaneous nitrification/denitrification (SND) can efficiently deplete NH by using air-exposed biocathode (AEB) in bioelectrochemical reactors. However, the fluctuation of wastewater adversely affects the functional biofilms and therefore the performance. In this work, four up-flow bioelectrochemical reactors (UBERs) with some novel inocula were investigated to improve domestic wastewater treatment.

Impact of low temperature on ex situ nitritation/in situ denitritation in field pilot-scale landfill for postclosure care of leachate treatment and gas content.

Leachates and landfill gas (LFG) are the major problems for closed landfills (CL) and cause significant threats to receiving waterbody and ambient air quality. In this study, a field pilot-scale CL with ex situ nitritation/in situ denitritation process was constructed and operated continuously under wide temperature variations. The effect of low temperature on leachate treatment, and LFG content was studied.

Comparative evaluation of simultaneous nitritation/denitritation and energy recovery in air-cathode microbial fuel cells (ACMFCs) treating low C/N ratio wastewater.

Air-cathode microbial fuel cells (ACMFCs) can extract available electrons from the low C/N ratio wastewater (LCNW) for pollutant degradation and power generation. However, the multiple effects of operating parameters and their relationship between the performances and parameters are still lacking. In this study, several ACMFCs for simultaneous nitritation/denitritation (SND) and energy recovery were constructed and evaluated in terms of chemical oxygen demand (COD), NH-N, C/N ratio, phosphate buffer solution (PBS), and external resistance (R), and several derived parameters (e.

One-pot degradation of urine wastewater by combining simultaneous halophilic nitrification and aerobic denitrification in air-exposed biocathode microbial fuel cells (AEB-MFCs).

Urine wastewater is used as fuel in microbial fuel cells to generate power for several applications. However, the knowledge on the removal efficiencies of pollutants and bacterial composition of electrode biofilm is still lacking. In this study, two air-exposed biocathode microbial fuel cells (AEB-MFCs) were constructed and some nitrogen-removing consortium were inoculated to fabricate multifunctional AEBs for urine treatment and energy recovery.

Bifunctional cathode using a biofilm and Pt/C catalyst for simultaneous electricity generation and nitrification in microbial fuel cells.

Biofouling frequently causes catalyst deterioration at the cathode of microbial fuel cells (MFCs). A biofilm-covered Pt/C cathode (BPC) was fabricated via in situ cultivation of a biofilm on a Pt/C cathode (PC) in a dual-chambered MFC, which enables effective removal of NH-N and copious generation of electricity. Experimental results show 99% NH-N removal by the nitrifying bacteria that constitute 35.

Risk assessment for and microbial community changes in Farmland soil contaminated with heavy metals and metalloids.

Food security and human health can be seriously affected by heavy metal and metalloid (HM) pollution of soil. In this study, the risks posed by HMs and microbial community responses to HM pollution of agricultural soil in southwestern China were investigated. The C, N, P, and S (nutrients) concentrations were 12040.

Effect of air-exposed biocathode on the performance of a Thauera-dominated membraneless single-chamber microbial fuel cell (SCMFC).

To investigate the effect of air-exposed biocathode (AEB) on the performance of single-chamber microbial fuel cell (SCMFC), wastewater quality, bioelectrochemical characteristics and the electrode biofilms were researched. It was demonstrated that exposing the biocathode to air was beneficial to nitrogen removal and current generation. In Test 1 of 95% AEB, removal rates of ammonia, total nitrogen (TN) and chemical oxygen demand (COD) reached 99.

Accuracy of Endorectal Endoscopic Ultrasound (EUS) for Locally Advanced Rectal Cancer (LARC) Restaging After Neoadjuvant Chemoradiotherapy (NAT): A Meta-Analysis.

Background/aims: Endorectal endoscopic ultrasound (EUS) can provide accurate and reliable information for initial staging of locally advanced rectal cancer (LARC) in both the depth of rectal cancer penetration (T-stage) and regional lymph node involvement (N-stage). However, there is still no consensus about its accuracy in retagging LARC after neoadjuvant chemoradiotherapy (NAT).

Methodology: In this study, we retrieved previous studies and performed a meta-analysis for assessing the accuracy of EUS for retagging of LARC after NAT.

The saci_2123 gene of the hyperthermoacidophile Sulfolobus acidocaldarius encodes an ATP-binding cassette multidrug transporter.

Multidrug resistance (MDR) transporters are capable of secreting structurally and functionally unrelated toxic compounds from the cell. Among this group are ATP-binding cassette (ABC) transporters. These membrane proteins are typically arranged as either hetero- or homo-dimers of ABC half-transporters with each subunit consisting of a membrane domain fused at the C-terminus to an ATP-binding domain, or as full transporters in which the two subunits are fused into a single polypeptide.

Deletion of cdvB paralogous genes of Sulfolobus acidocaldarius impairs cell division.

The majority of Crenarchaeota utilize the cell division system (Cdv) to divide. This system consists of three highly conserved genes, cdvA, cdvB and cdvC that are organized in an operon. CdvC is homologous to the AAA-type ATPase Vps4, involved in multivesicular body biogenesis in eukaryotes.