Effect of secretin and inhibitors of HCO3-/H+ transport on the membrane voltage of rat pancreatic duct cells.

The aim of the present study was to study the effect of secretin on the electrophysiological response of pancreatic ducts. Furthermore, we investigated the effects of lipid-soluble buffers and inhibitors of HCO3-/H+ transport. Ducts obtained from fresh rat pancreas were perfused in vitro.

Effect of vasoactive intestinal peptide, carbachol and other agonists on the membrane voltage of pancreatic duct cells.

The regulation of pancreatic exocrine secretion involves hormonal, neural and neurohormonal components. Many agonists are known to be effective in pancreatic acinar cells, but less is known about the ducts. Therefore, we wanted to investigate the influence of various agonists on isolated perfused pancreatic ducts and, as a physiological response, we measured the basolateral membrane voltage of the duct cells (Vbl) with microelectrodes.

[A vesico-sigmoid fistula in a 15-year-old girl].

The authors diagnosed in a fifteen-year-old girl. other wise healthy, a double vesicosigmoidal fistula. It developed probably at the age of two after an acute abdominal attack of inflammatory character which was treated conservatively.

Chloride and potassium conductances of cultured human sweat ducts.

The purpose of this study was to characterize the ion conductances, in particular those for Cl- and K+, of human sweat duct cells grown in primary culture. Sweat duct cells from healthy individuals were grown to confluence on a dialysis membrane, which was then mounted in a mini-Ussing chamber and transepithelial and intracellular potentials were measured under open-circuit conditions. Under control conditions the epithelia developed mucosa-negative transepithelial potentials, Vte, of about -10 mV.

Noninvasive estimation of total body water in critically ill children after cardiac operations. Validation of a bioelectric impedance method.

The understanding of fluid fluxes in pediatric cardiac critical care is crucial to effective management. Knowledge of variations in total body water in this situation would aid this understanding, but most available methods are unsuitable for routine use. Recently, estimation of total body water by a tetrapolar bioelectric impedance has been validated in older children and adolescents.

[Visual evoked potentials. Their use in ophthalmology and other specialties].

The technique of investigating evoked visual potentials (EVP) is resumed within the context of electrophysiological methods as applied in optic neuritis. Several typical courses of the disease are case-reported in this account. In conclusion, authors recommend further on EVP implications due to its objectivity, non-invasiveness and time-sparing with a possible automated evaluation.

[Epiglottitis and subglottic laryngitis].

Account of the course and conclusions of a working session of the Paediatric Society devoted to laryngeal obstruction in children. The participants discussed in particular the differential diagnosis of subglottic laryngitis and epiglottitis in children.

Effect of bicarbonate on potassium conductance of isolated perfused rat pancreatic ducts.

The aim of this study was to investigate the role of the K+ conductance in unstimulated and stimulated pancreatic ducts and to see how it is affected by provision of exogenous HCO3-/CO2. For this purpose we have applied electrophysiological techniques to perfused pancreatic ducts, which were dissected from rat pancreas. The basolateral membrane potential PDbl of unstimulated duct cells was between -60 mV and -70 mV, and the cells had a relatively large K+ conductance in the basolateral membrane as demonstrated by (a) 20-22 mV depolarization of PDbl in response to increase in bath K+ concentration from 5 mmol/l to 20 mmol/l and (b) the effect of a K+ channel blocker, Ba2+ (5 mmol/l), which depolarized PDbl by 30-40 mV.

[Hand, foot and mouth disease].

The authors described a case of viral stomatitis of the type of hand, foot and mouth disease with mucosal and skin manifestations in an adult with a significant rise of neutralizing antibodies against the Coxsackie B4 virus.

Perioperative monitoring of total body water by bio-electrical impedance in children undergoing open heart surgery.

Unlabelled: Knowledge of the changes in total body water (TBW) following cardiac surgery (OHS) in children would be of value in fluid therapy and in researching the causes and management of capillary leak. We have validated a bioelectrical impedance technique (BEI) for non-invasive estimation of TBW in children after OHS. We report the use of this method in a longitudinal study.

[Diagnosis and treatment of retrocaval ureter].

The authors present a brief characteristic of the genesis and development of a retrocaval ureter. They describe the case-history and treatment in a 3.5-year-old girl with right-sided hydronephrosis of unknown aetiology.

Cation transport by sweat ducts in primary culture. Ionic mechanism of cholinergically evoked current oscillations.

1. The coiled reabsorptive segment of human sweat ducts was cultured in vitro. Cells were then harvested and plated onto a dialysis membrane which was glued over a hole in a small disc.

Immune studies in human immunodeficiency virus infection with myasthenia gravis: a case report.

A patient with human immunodeficiency virus infection had cellular and humoral immune responses studied longitudinally from the onset of generalized myasthenia gravis. Progressive decline in CD4+, CD45R+ and CD4+, CDw29+ T-cells, cellular immune responses to alloantigen and mitogen stimulation, and acetylcholine receptor antibody titers were associated with clinical improvement of all myasthenic symptoms.

Acetate stimulates secretion in the rabbit mandibular gland.

In isolated perfused rabbit mandibular glands undergoing stimulation with 0.8 microM acetylcholine, replacement of HCO3- with acetate (25 mM) increased fluid secretion by more than 100%. Other short-chain fatty acids, except for propionate, had a similar effect.

Properties of the luminal membrane of isolated perfused rat pancreatic ducts. Effect of cyclic AMP and blockers of chloride transport.

The aim of the present study was to investigate by what transport mechanism does HCO-3 cross the luminal membrane of pancreatic duct cells, and how do the cells respond to stimulation with dibutyryl cyclic AMP (db-cAMP). For this purpose a newly developed preparation of isolated and perfused intra- and interlobular ducts of rat pancreas was used. Responses of the epithelium to inhibitors and agonists were monitored by electrophysiological techniques.

Electrophysiological study of transport systems in isolated perfused pancreatic ducts: properties of the basolateral membrane.

In order to study the mechanism of pancreatic HCO3- transport, a perfused preparation of isolated intra- and interlobular ducts (i.d. 20-40 microns) of rat pancreas was developed.

Effect of K+ and K+ channel blockers on pancreatic HCO3-transport.

The present study investigated whether there is a K+ conductive pathway on the basolateral membrane of pancreatic duct cells. Small inter- and intralobular ducts were isolated from rat pancreas and perfused in vitro. Duct cells were impaled and the potential across the basolateral membrane (PDbl) was measured.