Exploring newly isolated thermotolerant, halotolerant and antimicrobial resistant Bacillus subtilis ProNTL1 from tannery waste and its alkaline protease production.

Background: A hydrolytic enzyme called protease from microbial sources has expansive applications in leather as well as tannery industries and thus leaves no choice but to discover a potential candidate for efficient yield of protease with eccentric characteristics.

Methods And Results: In the current study, using skim milk agar medium, three bacterial strains (Pro SS14, Pro NTL1, Pro SM) were identified for protease production out of thirty tannery waste samples. The bacterial isolates were identified through morphological, biochemical and molecular basis and the highest protease producer (134 U/mL) having the paramount protein content (8.

Aflatoxin M1 detection by ELISA in raw and processed milk in Bangladesh.

An analysis was accomplished to get information regarding presence of highly toxic and carcinogenic aflatoxin M1 (AFM1) in raw and processed samples of milk applying ELISA (enzyme linked immunosorbent assay). Investigation of a set of 100 samples (n=100) taken from different regional small-scale farms as well as grocery stores of Bangladesh containing three groups of milk including raw (n=50), pasteurized (n=25) and UHT (n=25), exhibited in total 53% AFM1 contamination where 70% contamination was found in raw milk ranging from 22.79-1489.