Deciphering pathogenicity and virulence of the first isolate from diabetic foot osteomyelitis.

Introduction: This study identifies as a new coagulase-negative staphylococcal species isolated from diabetic foot osteomyelitis (DFOM) and provides an in-depth analysis of its pathogenic and virulence profile, as well as demonstrating its potential to cause infection.

Methods: The NSD001 strain was examined for its planktonic growth, biofilm production, and phagocytosis rates in murine macrophages compared to NSA739. Additionally, persistence and replication within human osteoblasts were investigated, while the zebrafish embryo model was employed to assess virulence.

The zebrafish embryo model: unveiling its potential for investigating phage therapy against methicillin-resistant infection.

is a pathogenic bacterium responsible for a broad spectrum of infections, including cutaneous, respiratory, osteoarticular, and systemic infections. It poses a significant clinical challenge due to its ability to develop antibiotic resistance. This resistance limits therapeutic options, increases the risk of severe complications, and underscores the urgent need for new strategies to address this threat, including the investigation of treatments complementary to antibiotics.

Isolation and Characterization of New Bacteriophages against Staphylococcal Clinical Isolates from Diabetic Foot Ulcers.

sp. is the most common bacterial genus in infections related to diabetic foot ulcers (DFUs). The emergence of multidrug-resistant bacteria places a serious burden on public health systems.

The ROSA-Like Prophage Colonizing Staphylococcus aureus Promotes Intracellular Survival, Biofilm Formation, and Virulence in a Chronic Wound Environment.

Background: The transition from colonization to invasion is critical in diabetic foot ulcer (DFU). Staphylococcus aureus can colonize DFU, or invade the underlying tissues, causing serious infections. The ROSA-like prophage has previously been implicated in strain colonization characteristics of S aureus isolates in uninfected ulcers.

Phenotypic and Genotypic Virulence Characterisation of Strains Isolated from Human Bloodstream and Diabetic Foot Infections.

is a recently described coagulase-negative identified in human diseases, especially in infections of foot ulcers in patients living with diabetes mellitus. To date, its pathogenicity remains underexplored. In this study, whole-genome analysis was performed on a collection of 29 clinical strains isolated from bloodstream and diabetic foot infections with regard to their phylogenetic relationships and comprehensive analysis of their resistome and virulome.

Characterization of the Secreted Acid Phosphatase SapS Reveals a Novel Virulence Factor of That Contributes to Survival and Virulence in Mice.

possesses a large arsenal of immune-modulating factors, enabling it to bypass the immune system's response. Here, we demonstrate that the acid phosphatase SapS is secreted during macrophage infection and promotes its intracellular survival in this type of immune cell. In animal models, the SA564 mutant demonstrated a significantly lower bacterial burden in liver and renal tissues of mice at four days post infection in comparison to the wild type, along with lower pathogenicity in a zebrafish infection model.

Bacteriophage Therapy for Infections: A Review of Animal Models, Treatments, and Clinical Trials.

() is a common and virulent human pathogen causing several serious illnesses including skin abscesses, wound infections, endocarditis, osteomyelitis, pneumonia, and toxic shock syndrome. Antibiotics were first introduced in the 1940s, leading to the belief that bacterial illnesses would be eradicated. However, microorganisms, including , began to develop antibiotic resistance from the increased use and abuse of antibiotics.

Investigating Pathogenicity and Virulence of An Emerging Pathogen.

is a coagulase-negative identified in 2002 that has been implicated in human diseases as an opportunistic pathogenic bacterium. Its multiresistant character is becoming a major health problem, yet the pathogenicity of is poorly characterized. In this study, the pathogenicity of a clinical isolate from diabetic foot osteomyelitis was compared with a strain in various in vitro and in vivo experiments.

Toxins: An Update on Their Pathogenic Properties and Potential Treatments.

is a clinically important pathogen that causes a wide range of human infections, from minor skin infections to severe tissue infection and sepsis. has a high level of antibiotic resistance and is a common cause of infections in hospitals and the community. The rising prevalence of community-acquired methicillin-resistant (CA-MRSA), combined with the important severity of infections in general, has resulted in the frequent use of anti-staphylococcal antibiotics, leading to increasing resistance rates.