Vitamin D Treatment Reverses the Induced Oxidative Stress Damage to DNA.

Background And Objective: The aim of the current study was to investigate in detail the effect of the active metabolite of vitamin D3 [1, 25 (OH)2 D3] in ameliorating the induced oxidative damage to DNA.

Materials And Methods: Primary cortical neuron cultures from one week old Wister rats were set up in sterile conditions. The neuron cultures were maintained for up to 72 h in culture in the presence of varying doses of vitamin D.

Recruitment and allosteric stimulation of a histone-deubiquitinating enzyme during heterochromatin assembly.

Heterochromatin formation in budding yeast is regulated by the silent information regulator (SIR) complex. The SIR complex comprises the NAD-dependent deacetylase Sir2, the scaffolding protein Sir4, and the nucleosome-binding protein Sir3. Transcriptionally active regions present a challenge to SIR complex-mediated heterochromatic silencing due to the presence of antagonistic histone post-translational modifications, including acetylation and methylation.