An acute toxicity study assessed the LC values for eight different amino acid ionic liquids (AAILs), featuring two cations, tetrabutylphosphonium [P] and tetrabutylammonium [N], coupled with four anions [PHE], [ASP], [SER], and [GLY]. According to the OECD 203 standard for acute fish toxicity tests with guppy fish (, all the AAILs exhibited low toxicity levels, and were practically nontoxic and harmless. The LC values surpassed 100 mg/L and 1000 mg/L.
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