Publications by authors named "Norma Arrigoni"

is diffused worldwide, and subsp. is spread worldwide with many serovars associated with the infection of domestic bovines. The most spread are .

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Background: The prolificacy of sows (litter size at birth) has markedly increased, leading to higher post-natal mortality. Heat stress can exacerbate this issue. Arginine plays an important role in several physiological pathways; its effect on gestating sows can depend on the period of supplementation.

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  • Paratuberculosis, or Johne's disease, is a contagious bacterial infection affecting ruminants, including certain wild and domestic animals, and is recognized under the new EU Animal Health Law requiring surveillance.
  • A study conducted in Stelvio National Park, Italy, from 2018 to 2022 analyzed 390 red deer for Mycobacterium avium subspecies paratuberculosis (MAP), finding a 5.9% prevalence overall, which declined over the years.
  • The research indicated that older deer and those with lower body mass were more likely to test positive for MAP, suggesting early stages of the infection with minimal signs of disease in the population.
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  • * This study compared two methods for assessing antimicrobial susceptibility in 215 bovine mastitis isolates: the broth microdilution (BMD) method and the agar disk diffusion (ADD) method, focusing on various common pathogens.
  • * Overall agreement between the BMD and ADD methods was 80.7%, indicating fair consistency, but specific pathogens like Staphylococcus aureus showed better agreement, while others like Streptococcus uberis and Escherichia coli had lower compatibility for certain antibiotics.
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Paratuberculosis is a notable infectious disease of ruminants. Goats appear to be particularly susceptible. The survey aimed to investigate the spread of paratuberculosis in Italian goat farming and evaluate whether the presence of the disease could be influenced by welfare and biosecurity deficiencies.

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Paratuberculosis is considered one of the most economically devastating infectious diseases of domestic livestock, and the most effective control strategy is a combination of 'test-and-cull' and on-farm biosecurity measures. In Italy, a Voluntary National Control Plan (VNCP) and guidelines have been introduced to reduce the impact of the disease, and farmers can voluntarily enroll in the control plan. The main aims of this study were: i) the description of the trend over a 4-year period on total, within-herd (WH) and between herd (BH) apparent seroprevalences observed in 64 dairy herds members of a mutual company located in Italy after the introduction of a proposed "Customized Control Plan" (CCP); ii) the evaluation of its effectiveness in terms of percentage of participating farms that decided to join the VNCP.

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Raw milk and dairy products are usually considered the major sources of subsp. (MAP) exposure for humans. During the production process of mozzarella cheese, as well as of other pasta-filata cheeses made with pasteurized or raw milk, curd is heated and stretched by addition of hot or boiling water.

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Paratuberculosis is a chronic enteric progressive disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP). Despite cultural methods being considered the gold standard for the diagnosis of paratuberculosis, quantitative PCR (qPCR) assays have been developed for this purpose.

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(group B Streptococcus, GBS) is a causative agent of mastitis in dairy cattle, mainly causing a subclinical disease associated with a high somatic cell count (SCC), and a consequent decrease in production yield and quality of milk. GBS has been almost eradicated in many Northern European countries, but there are warnings of its re-emergence as a zoonotic threat. In Italy, only two regions carry out a GBS control program: Lombardy and Emilia-Romagna.

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We report here on an outbreak of mastitis caused by Streptococcus agalactiae, or group B Streptococcus, in a northern Italy (Lombardy Region) free stall dairy farm. This outbreak was unusual because it occurred in a closed dairy herd and proved to be extremely difficult to resolve even after the application of the classical control procedures, which are specifically focused on the contagious nature of S. agalactiae.

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(group B , GBS) is one of the most important agents of bovine mastitis and causes remarkable direct and indirect economic losses to the livestock sector. Moreover, this species can cause severe human diseases in susceptible individuals. To investigate the zoonotic potential of , 203 sympatric isolates from both humans and cattle, isolated in the same time frame (2018) and in the same geographic area (Emilia Romagna region, Northern Italy), were characterized by molecular capsular typing (MCT), pilus island typing (PI), and multi-locus sequence typing (MLST).

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The cattle industry is a major driving force for the Italian agricultural sector totalling about 5. 6 million heads for dairy and meat production together. It is particularly developed in the northern part of the country, where 70% of the whole Italian cattle population is reared.

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Paratuberculosis, a chronic disease caused by subsp. (MAP), in ten scimitar-horned oryxes (SHOs) hosted in an Italian zoological park and originating from a Slovakian flock, was documented by pathology, molecular, cultural, and serological testing. The infection origin in this threatened species was also investigated by genomic analyses.

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  • Paratuberculosis is an infectious disease in ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP), which may also have implications for human health due to its association with Crohn's disease.
  • After detection of the disease, there is no cure available and culling infected animals is the only way to control its spread, making early detection crucial.
  • The study uses an immunoinformatic approach to analyze 10 MAP proteins for their immune response potential, aiming to develop better diagnostic tools and vaccine formulations.
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The aim of this study was to develop and validate different innovative DNA extraction methods to detect Mycobacterium avium subsp. paratuberculosis (MAP) DNA from bovine and buffalo colostrum. Paratuberculosis is a chronic inflammatory infection of domestic and wild animals, especially ruminants, caused by MAP.

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Paratuberculosis, a chronic disease affecting ruminant livestock, is caused by Mycobacterium avium subsp. paratuberculosis (MAP). It has direct and indirect economic costs, impacts animal welfare and arouses public health concerns.

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  • Paratuberculosis, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic infection affecting ruminants and is linked to diseases like Crohn's in humans.
  • This study evaluated the performance of three methods—culturing, quantitative PCR (qPCR), and a peptide-mediated magnetic separation (PMS) phage-based assay—for detecting MAP in various milk types, like raw and powdered.
  • Results showed that qPCR (94% sensitivity) and culture (83% sensitivity) were more effective than the PMS-phage method (40% sensitivity), indicating that qPCR and culture are the best options for MAP detection in dairy products.
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Background and aimEpidemiology of human non-typhoid salmonellosis is characterised by recurrent emergence of new clones of the pathogen over time. Some clonal lines of have shaped epidemiology of the disease at global level, as happened for serotype Enteritidis or, more recently, for 4,[5],12:i:-, a monophasic variant of serotype Typhimurium. The same clonal behaviour is recognisable at sub-serotype level where single outbreaks or more generalised epidemics are attributable to defined clones.

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  • Paratuberculosis, caused by Mycobacterium avium subsp. paratuberculosis (MAP), primarily affects ruminants like red deer and was studied during a culling program in Stelvio National Park (2011-2015).
  • Out of 484 samples tested, including tissue and fecal specimens, a significant portion tested PCR positive, indicating the presence of the disease in both the Lombardy and Bolzano regions.
  • All MAP strains isolated had a similar genetic profile, providing evidence of a common origin and suggesting potential transmission of the disease between wildlife and domestic livestock in Northern Italy.
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In Europe, Trichinella spiralis, the most dangerous species for humans of the genus Trichinella, has a patchy distribution with important foci in Eastern countries and Spain. This zoonotic pathogen was apparently not circulating among wild and domestic animals of Italy. In 2016, muscle larvae belonging to this nematode species were detected in a red fox (Vulpes vulpes) shot in the Piacenza province (Northern Italy).

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Consumption of milk and dairy products is considered one of the main routes of human exposure to Mycobacterium avium subsp. paratuberculosis (MAP). Quantitative data on MAP load in raw cows' milk are essential starting point for exposure assessment.

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  • * Two separate RA models were created: one for boiled milk and one for raw milk, with predictions showing that no cases occurred under optimal storage conditions, but risks varied significantly with raw milk depending on storage conditions and consumer demographics (especially age).
  • * The model highlighted the importance of quantifying risks related to raw milk consumption for public health, emphasizing the need for targeted educational programs to promote safety and inform consumer behavior.
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Two quantitative risk assessment (RA) models were developed to describe the risk of salmonellosis and listeriosis linked to consumption of raw milk sold in vending machines in Italy. Exposure assessment considered the official microbiological records monitoring raw milk samples from vending machines performed by the regional veterinary authorities from 2008 to 2011, microbial growth during storage, destruction experiments, consumption frequency of raw milk, serving size, and consumption preference. Two separate RA models were developed: one for the consumption of boiled milk and the other for the consumption of raw milk.

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Mycobacterium avium subsp. paratuberculosis (MAP) is the cause of a chronic enteritis of ruminants (bovine paratuberculosis (PTB)--Johne's disease) that is associated with enormous worldwide economic losses for the animal production. Diagnosis is based on observation of clinical signs, the detection of antibodies in milk or serum, or evaluation of bacterial culture from feces.

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