Identification of Transgene-Free CRISPR-Edited Plants of Rice, Tomato, and by Monitoring DsRED Fluorescence in Dry Seeds.

Efficient elimination of the editing machinery remains a challenge in plant biotechnology after genome editing to minimize the probability of off-target mutations, but it is also important to deliver end users with edited plants free of foreign DNA. Using the modular cloning system Golden Braid, we have included a fluorescence-dependent transgene monitoring module to the genome-editing tool box. We have tested this approach in , , and .