New side-view imaging technique for observing posterior chamber structures during cataract surgery in porcine eyes.

Background: To develop a side-view imaging technique for observing the dynamic behavior of posterior chamber structures (PCSs) in porcine eyes which mimics closed-eye cataract surgery in humans.

Methods: Enucleated porcine eyes were placed into liquid nitrogen for 5 seconds and immediately bisected at about a 45-degree angle to the equatorial plane. The anterior portion was attached firmly to a glass slide with superglue and sprinkled with wheat flour.

Disruption of the posterior chamber-anterior hyaloid membrane barrier during phacoemulsification and aspiration as revealed by contrast-enhanced magnetic resonance imaging.

Objective: To determine the effect of phacoemulsification and aspiration (PEA) in different surgical settings on the posterior chamber-anterior hyaloid membrane (AHM) barrier.

Methods: Eighty enucleated porcine eyes divided into 8 groups underwent lens extraction at a bottle height of 35 or 95 cm by 1 of the following 4 procedures: standard PEA, standard PEA without hydrodissection (sPEA-), sPEA- including 5 minutes of irrigation of the anterior chamber, and sPEA- including 20 cycles of deflation (5 seconds each cycle) and inflation (10 seconds each cycle) of the anterior chamber. Distribution of gadopentetate dimeglumine (gadolinium-diethylenetriamine pentaacetic acid [Gd-DTPA]) in the irrigating fluid was assessed by magnetic resonance imaging (MRI).