Cucurbitacin D isolated from Trichosanthes kirilowii induces apoptosis in human hepatocellular carcinoma cells in vitro.

The aim of the present study is to examine the effects of the anti-tumor component isolated from Trichosanthes kirilowii on human hepatocellular carcinoma cells. Using Sephadex G-25 column chromatography, Sep-Pak Plus C18 cartridge and high-performance liquid chromatography (HPLC), we isolated the active component from trichosanthes extract. By fast atom bombardment mass spectrometric analysis, the molecular mass of the active fraction was determined, the active components identified, and their mechanisms of action were analyzed by cell growth assay, cell cycle analysis, TUNEL staining and Western blot analysis.

[Ethical opinion survey on the periodical health examination among workers: privacy protection and informed consent on the information of the heath examination among workers in small and medium-sized enterprises].

We investigated the ethical opinions concerning the information on legal regular health examinations with a questionnaire for 757 workers in enterprises that contracted a part-time occupational physician as there were no full-time occupational health professionals. Thirty to 50% of the workers answered that the information on their health examination was known to their superiors and their colleagues. Also, 20 to 40% of the workers answered that the information was made known to other people without obtaining their agreement.