Cultivation and mating of the truffle in plantations of ectomycorrhizal seedlings.

Unlabelled: , a white-colored truffle that is endemic to Japan, is promising for culinary purposes due to its unique aroma. We were able to cultivate in plantations of inoculated seedlings for the first time. Ascocarps were found after 43 months at one site and after 61 months at another.

Mitospore formation on pure cultures of Tuber japonicum (Tuberaceae, Pezizales) in vitro.

The members of the genus Tuber are Ascomycota that form ectomycorrhizal associations with various coniferous and broadleaf tree species. In the teleomorphic stage, the species of the genus produce fruit bodies known as true truffles. Recent studies have discovered mitosporic structures, including spore mats, of several Tuber species on forest soils, indicating the presence of a cryptic anamorphic stage or an unknown reproductive strategy.

Physiological characteristics of pure cultures of a white-colored truffle .

A white-colored truffle , indigenous to Japan, is an ascomycetous ectomycorrhizal fungus. To clarify the physiological characteristics of this fungus, we investigated the influence of culture medium, temperature, and sources of nitrogen (N) and carbon (C) on the growth of five strains. strains grew better on malt extract and modified Melin-Norkrans medium, and showed peak growth at 20 °C or 25 °C.

Inside Cover Image, Volume 8, Issue 1.

The cover image is based on the ORIGINAL RESEARCH Tuber japonicum by Tomoko Shimokawa et al., https://doi.org/10.

Component features, odor-active volatiles, and acute oral toxicity of novel white-colored truffle native to Japan.

Component analysis of a novel white-colored truffle native to Japan, , was performed to determine its characteristic features. The analysis of odor-active volatile compound showed a high contribution of 1-octen-3-ol and 3-methyl-2,4-dithiapentane to the odor of . Although 2,4-dithiapentane is a key odorant of well-known white truffle , 3-methyl-2,4-dithiapentane was detected from the ripe .

Localization of helotialean fungi on ectomycorrhizae of Castanopsis cuspidata visualized by in situ hybridization.

Non-ectomycorrhizal fungi that associate with typical ectomycorrhizae often remain hidden, and their localization inside ectomycorrhizal (ECM) roots has remained uncharacterized. In this study, the fungal community associated with the ectomycorrhizae of Castanopsis cuspidata was investigated using a culture-dependent isolation technique. Additionally, the species composition and localization were determined using molecular techniques.