Publications by authors named "Norihito Miyake"
The mechanical strength of sarcomere structures of skeletal muscle was studied by rupturing single myofibrils of rabbit psoas muscle by submicromanipulation techniques. Microbeads coated with alpha-actinin were attached to the surface of myofibrils immobilized to coverslip. By use of either optical tweezers or atomic force microscope, the attached beads were captured and detached from the myofibrils.
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- The study explored how myosin filaments move along actin filaments using laser tweezers to manipulate single actin filaments suspended between microbeads.
- Results showed that when myosin-coated beads were dragged, they slid along the actin at 3-5 micrometers per second, initially moving in zigzag patterns.
- It was concluded that myosin-actin interactions are weak and affected by thermal agitation, but these effects can be minimized by mechanical constraints, with the sliding force acting almost parallel to the filament axes.