Malaria infection impairs glucuronidation and biliary excretion by the isolated perfused rat liver.

1. The effect of the erythrocyte stage of malaria infection on hepatic glucuronidation, biliary excretion and oxidation processes was investigated using harmol, salbutamol, taurocholate and propranolol. Livers from rats infected with the rodent malaria parasite P.

[Hepatitis C virus antibody in Hungarian blood donors, persons at risk and patients with post-transfusion hepatitis].

The authors screened blood donors, persons at high risk of infection and patients with post-transfusion hepatitis by the use of the new hepatitis C antibody test. 1.7% of persons donating blood acceptable for use according to current criteria were found positive.

Immunohistochemical localization of raf protein kinase in dendritic spines and spine apparatuses of the rat cerebral cortex.

The ultrastructural localization of raf protein (product of the raf protooncogene) in the neocortex, pyriform cortex and hippocampus of the rat has been investigated by means of pre-embedding immunohistochemistry. Specificity of the antiserum was tested with Western blotting. Besides the immunoreactivity of the dendrites, remarkably strong immunostaining of the dendritic spines and spine apparatuses was noted in each of the investigated areas.

Disposition of the diastereoisomers quinine and quinidine in the ovine fetus.

The disposition of the diastereoisomers quinine and quinidine was investigated in the near-term pregnant ewe. Five sheep were administered quinine and quinidine separately in random order by a combination of bolus and 30-h iv infusion. On a subsequent occasion, four of the five sheep were also administered the two drugs simultaneously.

Time dependent loss of tissue GABA content and immunoreactivity in hippocampal slices.

Immunohistochemical detection of GABA was used to evaluate changes of the GABA innervation in hippocampal slices maintained in vitro. In parallel experiments the amount of GABA, glutamate and aspartate was measured with high performance liquid chromatography. The results showed that while glutamate and aspartate levels remained fairly constant, GABAergic neurons suffered remarkable alterations.

Oral bioavailability of omeprazole before and after chronic therapy in patients with duodenal ulcer.

1. This study investigated the mechanism of the increase in oral bioavailability of omeprazole during repeated oral dosing. Eight patients with duodenal ulcer received an i.

Induction of raf-1 protein immunoreactivity in guinea pig hippocampal slices during the in vitro maintenance.

The cellular raf-1 protooncogene encodes a cytoplasmic serine/threonine protein kinase; raf-1 is expressed not only in tumour cells, but also in normal brain tissue. However, the substrate of raf-1 kinase is unknown and its function is still hypothetical. In cell cultures, mitogen stimulation may alter its properties, including its kinase activity and subcellular distribution.

Expression and quantitative changes of carbonic anhydrase in developing neurones of rat central nervous system.

Postnatal changes in carbonic anhydrase activity were investigated in the islands of Calleja, which have been previously reported to contain the enzyme. Results obtained with a new modified method of Hansson provided further evidence for the distinction between the medial and lateral islands of Calleja. The enzyme was localized mainly in the nucleus and cytoplasm of granule cells without showing binding to any cytoplasmic organelle.

Ontogeny of fetal renal organic cation excretion: a study with cimetidine and ranitidine during the latter half of gestation in the pregnant ewe.

The organic cation cimetidine undergoes renal tubular secretion in the near-term ovine fetus. We investigated the ontogeny of renal tubular secretion of organic cations in the fetus from 80 days of gestation (term = 145). Sixteen sheep were administered both cimetidine and ranitidine in random order by a combination of bolus and i.

Pharmacokinetics and toxicity of two schedules of high dose epirubicin.

Epirubicin, a stereoisomer of doxorubicin, is reported to have equal antitumor activity with lower cardiac and systemic toxicity. Recently the maximum tolerated dose of this drug has been revised upwards with reported increased response rates. However, the pharmacokinetics of epirubicin at high doses have never been reported.

Transient increase of raf protein kinase-like immunoreactivity in the rat dentate gyrus during long-term potentiation.

Altered levels of cellular raf proteins (products of the raf protooncogenes) have been shown in the neurons of the dentate fascia of rats in response to high-frequency stimulation, with light microscopic immunohistochemistry by using polyclonal antibodies. No raf-1-like staining was seen in unstimulated tissue, while the pan-raf antibodies revealed immunoreactivity in the cytoplasm of neurons in the Ammon's horn and dentate fascia of rats and guinea pigs. The induction of long-term potentiation in the dentate fascia of freely-moving rats triggered the appearance of raf-1-like staining and increased the number of granule cells with pan-raf-like immunoreactivity.