Recovery of preservation-injured primary human hepatocytes and nonparenchymal cells to tissuelike structures in large-scale bioreactors for liver support: an initial transmission electron microscopy study.

This study investigated large-scale regeneration and tissue reorganization of adult human liver cells from preservation injured transplant organs. The use of basement membrane protein gels and growth factor enriched culture medium in standard culture flasks promotes liver tissue formation in isolated rat and pig hepatocytes, resulting in prolongation of phenotypic stability and metabolic competence of primary cells in vitro. A special bioreactor construction for high-density three-dimensional cell recovery was developed and isolation of cells from discarded human donor livers was enabled.

Electron microscopic and immunohistochemical examination of scarred human cornea re-treated by excimer laser.

Purpose: To elucidate differences, at the macromolecular level, in corneal tissue subjected to repeated argon fluoride excimer treatment.

Methods: A light microscopic, electron microscopic, and immunohistochemical study was performed on a scarred human cornea.

Results: Keratocytes were enlarged with an expanded endoplasmic reticulum and exhibited a fibroblastic appearance.