Determination of aflatoxin M in milk and dairy products using high performance liquid chromatography-fluorescence with post column photochemical derivatization.

The determination of aflatoxin M in milk using high performance liquid chromatography with photochemical post-column derivatization and fluorescence detection is described. The samples were first extracted and clean-up using the immunoaffinity AFLATEST column originally targeted for aflatoxins B, B, G and G. The separation of aflatoxin M were performed using C18 Hypersil gold (150mm×4.

Natural occurrence of aflatoxins and ochratoxin A in processed spices marketed in Malaysia.

The analysis of aflatoxins (B1, B2, G1 and G2) and ochratoxin A (OTA) was performed in processed spices marketed in Penang, Malaysia, using immunoaffinity columns and HPLC equipped with fluorescence detector (HPLC-FD). The processed powdered spices analysed include dried chilli, fennel, cumin, turmeric, black and white pepper, poppy seed, coriander, 'garam masala', and mixed spices for fish, meat and chicken curry. Two different studies were carried out.