Cytotoxicity of Fast-set Conventional and Resin-modified Glass Ionomer Cement Polymerized at Different Times on SHED.

Objectives: To compare the cytotoxicity of conventional GIC and Resin Modified GIC (RMGIC) polymerized at 2 different times on stem cells from human exfoliated deciduous teeth (SHED).

Study Design: The conventional GIC (Fuji IX GP Extra) and RMGIC (Fuji II LC) were mixed and incubated in a prepared Dublecco's Modified Eagle Medium (DMEM) for seven days. After seeding the characterized SHED for 24 hrs, six replicates of seven serially diluted extracts of each group were added and incubated for 72 hrs.

Effects of Perivitelline Fluid Obtained from Horseshoe Crab on The Proliferation and Genotoxicity of Dental Pulp Stem Cells.

Objective: Perivitelline fluid (PVF) of the horseshoe crab embryo has been reported to possess an important role during embryogenesis by promoting cell proliferation. This study aims to evaluate the effect of PVF on the proliferation, chromosome aberration (CA) and mutagenicity of the dental pulp stem cells (DPSCs).

Materials And Methods: This is an in vitro experimental study.

Proliferation rate of stem cells derived from human dental pulp and identification of differentially expressed genes.

Stem cells from human exfoliated deciduous teeth (SHED) and dental pulp stem cells (DPSCs) obtained from the dental pulp of human extracted tooth were cultured and characterized to confirm that these were mesenchymal stem cells. The proliferation rate was assessed using AlamarBlue® cell assay. The differentially expressed genes in SHED and DPSCs were identified using the GeneFishing™ technique.

Physical properties and cytotoxicity comparison of experimental gypsum-based biomaterials with two current dental cement materials on L929 fibroblast cells.

Aim: To evaluate physical properties and cytotoxicity of pure gypsum-based (pure-GYP) and experimental gypsum-based biomaterials mixed with polyacrylic acid (Gyp-PA). The results were compared with calcium hydroxide (CH) and glass ionomer cement (GIC) for application as base/liner materials.

Materials And Methods: Vicat's needle was used to measure the setting time and solubility (%) was determined by percentage of weight loss of the materials following immersion in distilled water.

Cytotoxicity of accelerated white MTA and Malaysian white Portland cement on stem cells from human exfoliated deciduous teeth (SHED): An in vitro study.

The aim of this study was to compare the cytotoxicity of accelerated-set white MTA (AWMTA) and accelerated-set Malaysian white PC (AMWPC) on stem cells from human exfoliated deciduous teeth (SHED). The test materials were introduced into paraffin wax moulds after mixing with calcium chloride dihydrate and sterile distilled water. Subsequently, the set cement specimens were sterilized, incubated in a prepared Dulbecco's modified Eagle medium (DMEM) for seven days.

Cytotoxicity evaluation of a new fast set highly viscous conventional glass ionomer cement with L929 fibroblast cell line.

Aim: This study aims to evaluate the cytotoxicity of a new fast set highly viscous conventional glass ionomer cement (GIC) with L929 fibroblasts.

Materials And Methods: The cement capsule was mixed and introduced into a paraffin wax mould. After setting, the cement was incubated in Dulbecco's Modified Eagle's Medium.

In vitro cytotoxic evaluation of processed natural coral in human osteoblasts.

This study aimed to evaluate the in vitro cytotoxic effects of locally produced processed natural coral (PNC) using human osteoblasts (HOS). Cytotoxicity was not observed when HOS cells were cultured with PNC, as assessed by (3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyl tetrazolium bromide; MTT) and Neutral Red (NR) assays at concentration up 200 mg/mL for up to 72 hours. Flow cytometry (FCM) analysis showed that PNC (200 mg/mL) did not decrease viability of HOS cells after 48 and 72 hours of treatment.

The microscopic biological response of human chondrocytes to bovine bone scaffold.

This study was performed to determine the microscopic biological response of human nasal septum chondrocytes and human knee articular chondrocytes placed on a demineralized bovine bone scaffold. Both chondrocytes were cultured and seeded onto the bovine bone scaffold with seeding density of 1 x 105 cells per 100 microl/scaffold and incubated for 1, 2, 5 and 7 days. Proliferation and viability of the cells were measured by mitochondrial dehydrogenase activity (MTT assay), adhesion study was analyzed by scanning electron microscopy and differentiation study was analyzed by immunofluorescence staining and confocal laser scanning electron microscopy.