Species-specific PCR for the Diagnosis and Determination of Antibiotic Susceptibilities of Strains Isolated from Tehran, Iran.

Background: Brucellosis is an endemic zoonotic disease in the Middle East. This study intended to design a uniplex PCR assay for the detection and differentiation of at the species level and determining the antibiotic susceptibility pattern of in Iran.

Methods: Sixty-eight Brucella specimens (38 animal and 30 human specimens) were analyzed using PCR (using one pair of primers).