Immunodominance of LipL32 peptides revealed by leptospirosis sera and therapeutic monoclonal antibodies.

Background/purpose: Leptospirosis is a neglected zoonosis, imposing significant human and veterinary public health burdens. In this study, recombinant LipL32 and LipL32 middle domain of LipL32, and LipL32, and LipL32 of c-terminal LipL32 truncations were defined for immunodominance of the molecule during Leptospira infections revealed by leptospirosis sera.

Results: IgM-dominant was directed to highly surface accessible LipL32 and Lipl32.

The saposin-like proteins 1, 2, and 3 of Fasciola gigantica.

The SAP genes of Fasciola encode proteins belonging to the saposin-like protein family. The saposin signature, a compact domain of mainly alpha-helical character, contains six conserved cysteine residues and has been implicated in membrane-binding, pore formation, and subsequent cell lysis in several family members. Recombinant SAP-2 of F.