Nano magnetic-based ELISA and nano magnetic-based latex agglutination test for diagnosis of experimental trichinellosis.

Human trichinellosis is a worldwide foodborne public health threat. Detecting circulating antigens of "" allows for an early diagnosis before larval encystation develops in skeletal muscles. For the first time, the present study aimed to formulate an effective nanomagnetic beads based-ELISA and -latex agglutination test (NMB-ELISA and NMB-LAT) to recognize adult worm crude extract antigen (AWCEA) in sera of experimentally infected mice.

Insecticidal, Oxidative, and Genotoxic Activities of Syzygium aromaticum and Eucalyptus globulus on Culex pipiens Adults and Larvae.

Objective: The wide-reaching Culex pipiens has long been a public apprehension. Combating serious vector-borne diseases requires the use of insecticides effective against both humans and the ecosystem. The wide variation of botanicals that nature has to offer tempts researchers to study their interactions with the insects.

Bioefficacy of Some Egyptian Aromatic Plants on (Diptera: Culicidae) Adults and Larvae.

Background: Protecting the environment from chemical hazards of synthetic insecticides along with offering of new breeding areas for vectors by urbanization indicate the trial of natural insecticides.

Methods: The acetone extracts of , and were tested for their insecticidal effect on adults and larvae in different concentrations depending on the technique used.

Results: The extracts were significantly effective in all models used with basil being the best in all tested three techniques (LC= 0.

Musca domestica laboratory susceptibility to three ethnobotanical culinary plants.

Throughout history, synanthropic Musca domestica had remained a worldwide problem whenever poor sanitation and bad hygienic conditions exists. Houseflies growing resistance to chemical insecticides are a rising environmental problem that necessitates search for alternatives. Mentha cervina, Ocimum basilicum, and Coriandrum sativum were tested for bioactivity on M.

Potential correlation between carboxylic acid metabolites in Biomphalaria alexandrina snails after exposure to Schistosoma mansoni infection.

Carboxylic acids play an important role in both aerobic and anaerobic metabolic pathways of both the snail and the parasite. Monitoring the effects of infection by schistosome on Biomphalaria alexandrina carboxylic acids metabolic profiles represents a promising additional source of information about the state of metabolic system. We separated and quantified pyruvic, fumaric, malic, oxalic, and acetic acids using ion-suppression reversed-phase high performance liquid chromatography (HPLC) to detect correlations between these acids in both hemolymph and digestive gland gonad complex (DGG's) samples in a total of 300 B.

Carboxylic acids as biomarkers of Biomphalaria alexandrina snails infected with Schistosoma mansoni.

Biomphalaria alexandrina snails play an indispensable role in transmission of schistosomiasis. Infection rates in field populations of snails are routinely determined by cercarial shedding neglecting prepatent snail infections, because of lack of a suitable method for diagnosis. The present study aimed at separation and quantification of oxalic, malic, acetic, pyruvic, and fumaric acids using ion-suppression reversed-phase high performance liquid chromatography (HPLC) to test the potentiality of these acids to be used as diagnostic and therapeutic biomarkers.