Mono-(2-ethylhexyl) phthalate (MEHP) affects intercellular junctions of Sertoli cell: A potential role of oxidative stress.

We analyzed the potential role of oxidative stress induced by mono (2-ethylhexyl) phthalate (MEHP) in adherent cell junction protein expression of prepubertal rat Sertoli cells (SC) in vitro. Five-day SC cultures were treated with MEHP (200μM) for 24h and compared to cells in basal conditions. Western blot and immunofluorescent (IF) analyses showed that MEHP induced increase of N-cadherin and catenin expression, modifying its distribution.

Effects of exposure to Di-(2-ethylhexyl) phthalate (DEHP) during lactation and puberty on sexual maturation and glycemic homeostasis in males rats.

This study aimed to evaluate the effects of Di-(2-ethylhexyl) phthalate (DEHP) exposure during lactation or puberty on the glycemic homeostasis and the sexual maturation of male rats. Thus, in a first experiment, Wistar rats were exposed to DEHP (7.5 mg/kg/day and 75 mg/kg/day) from the 1st to the 21st day of lactation.