Low Silver/Copper Exchange in a Copper-Phosphate Enzyme Nanoflower Hybrid Extremely Enhanced Antimicrobial Efficacy against Multidrug Resistant Bacteria.

Infections caused by bacteria that are resistant to many drugs are a major threat to public health in many countries around the world. Here we demonstrate the creation of heterogeneous catalytic nanomaterials with outstanding antimicrobial properties against several superbugs. We have shown that replacing a small amount of copper in a generated copper-phosphate-enzyme nanoflower hybrid with silver drastically increases the antimicrobial capacity of the nanomaterial.

Copper(i) as a reducing agent for the synthesis of bimetallic PtCu catalytic nanoparticles.

This work investigates the potential utilization of Cu(i) as a reducing agent for the transformation of the platinum salt KPtCl, resulting in the production of stable nanoparticles. The synthesized nanoparticles exhibit a bimetallic composition, incorporating copper within their final structure. This approach offers a convenient and accessible methodology for the production of bimetallic nanostructures.

Design and Synthesis of Copper Nanobiomaterials with Antimicrobial Properties.

In this work, nanostructured copper materials have been designed, synthetized, and evaluated in order to produce a more efficient and sustainable copper bionanohybrid with catalytical and antimicrobial properties. Thus, conditions are sought where the most critical steps are reduced or minimized, such as the use of reducing agents or the cryogenization step. In addition, the new materials have been characterized through different techniques, and their oxidative and reductive capacities, as well as their antimicrobial activity, have been evaluated.

Preparation of Highly Stable and Cost-Efficient Antiviral Materials for Reducing Infections and Avoiding the Transmission of Viruses such as SARS-CoV-2.

The current global pandemic due to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus has demonstrated the necessity to develop novel materials with antimicrobial and antiviral activities to prevent the infection. One significant route for the spread of diseases is by the transmission of the virus through contact with contaminated surfaces. Antiviral surface treatments can help to reduce or even avoid these hazards.

Graphene-TLL-CuONPs Hybrid as Highly Efficient Catalyst for Degradation of Organic Compounds.

In this work, CuO nanoparticles (NPs) were created in situ on graphene functionalized with lipase (G@TLL) where site-oriented supported TLL acted as template and binder in the presence of copper salt by tailorable synthesis under mild conditions, producing a heterogeneous catalyst. CuO NPs were confirmed by XRD and XPS. The TEM microscopy showed that the nanoparticles were homogeneously distributed over the G@TLL surface with sizes of 53 nm and 165 nm.

Temperature-induced formation of Pd nanoparticles in heterogeneous nanobiohybrids: application in C-H activation catalysis.

The effect of the temperature in the synthesis of Pd nanoparticles in the metal-enzyme biohybrids is evaluated. The effect on the formation, size, and morphology of nanoparticles was evaluated using B lipase as the protein scaffold. XRD analyses confirmed the formation of crystalline Pd(0) as the metal species in all cases.

Cascade Catalysis Through Bifunctional Lipase Metal Biohybrids for the Synthesis of Enantioenriched O-Heterocycles from Allenes.

Lipase/metal nanobiohybrids, generated by growth of silver or gold nanoparticles on protein matrixes are used as highly effective dual-activity heterogeneous catalysts for the production of enantiomerically enriched 2,5-dihydrofurans from allenic acetates in a one-pot cascade process combining a lipase-mediated hydrolytic kinetic resolution with a metal-catalyzed allene cycloisomerization. Incorporating a novel strategy based on enzyme-polymer bioconjugates in the nanobiohybrid preparation enables excellent conversions in the process. lipase B (CALB) in combination with a dextran-based polymer modifier (DexAsp) proved to be most efficient when merged with silver nanoparticles.

Synthesis of silver and gold nanoparticles-enzyme-polymer conjugate hybrids as dual-activity catalysts for chemoenzymatic cascade reactions.

Novel hybrids containing silver or gold nanoparticles have been synthesized in aqueous media and at room temperature using enzymes or tailor-made enzyme-polymer conjugates, which directly induced the formation of inorganic silver or gold species. The choice of pH, protein, or bioconjugate strongly affected the final metallic nanoparticles hybrid formation. Using (CALB) in a solution, nanobiohybrids containing AgO nanoparticles of 9 nm average diameter were obtained.

Glyconanomaterials for Human Virus Detection and Inhibition.

Viruses are among the most infectious pathogens, responsible for the highest death toll around the world. Lack of effective clinical drugs for most viral diseases emphasizes the need for speedy and accurate diagnosis at early stages of infection to prevent rapid spread of the pathogens. Glycans are important molecules which are involved in different biological recognition processes, especially in the spread of infection by mediating virus interaction with endothelial cells.

Enzyme/Nanocopper Hybrid Nanozymes: Modulating Enzyme-like Activity by the Protein Structure for Biosensing and Tumor Catalytic Therapy.

Artificial enzymes with modulated enzyme-mimicking activities of natural systems represent a challenge in catalytic applications. Here, we show the creation of artificial Cu metalloenzymes based on the generation of Cu nanoparticles in an enzyme matrix. Different enzymes were used, and the structural differences between the enzymes especially influenced the controlled the size of the nanoparticles and the environment that surrounds them.

Fast Degradation of Bisphenol A in Water by Nanostructured CuNPs@CALB Biohybrid Catalysts.

Copper nanoparticles-enzyme biohybrid is a promising material for the remediation of contaminated waters, but its function is influenced by its effect on degradation organic pollutants. This study is the first investigation into the fast degradation of a high amount of Bisphenol A (BPA) in water at neutral pH and room temperature. Four different CuNPs biohybrids with different cu species and nanoparticle sizes were used as catalysts.

Efficient Production of Multi-Layer Graphene from Graphite Flakes in Water by Lipase-Graphene Sheets Conjugation.

Biographene was successfully produced in water from graphite flakes by a simple, rapid, and efficient methodology based on a bioexfoliation technology. The methodology consisted in the application of a lipase, with a unique mechanism of interaction with hydrophobic surfaces, combined with a previous mechanical sonication, to selectively generate lipase-graphene sheets conjugates in water at room temperature. The adsorption of the lipase on the graphene sheets permits to keep the sheets separated in comparison with other methods.