Intraspecies interactions of impact biofilm architecture and virulence determinants in childhood dental caries.

Early childhood dental caries (ECC) is the most common chronic disease among children, especially among low socioeconomic populations. is most frequently associated with initiation of ECC. Although many studies report children with multiple strains (i.

Intraspecies interactions of impact biofilm architecture and virulence determinants in childhood dental caries.

Early childhood dental caries (ECC) is the most common chronic disease among children with a heavy disease burden among low socioeconomic populations. is most frequently associated with initiation of ECC. Many studies report children with multiple strains (i.

Body Mass Index and Early Childhood Caries in High Caries Risk Children: A Nested Case-Control Methodological Investigation.

To evaluate body mass index (BMI) - early childhood caries (ECC) relationships with various BMI expressions.
Healthy eight- to 18-month children with unerupted molar(s) were conveniently sampled from Uniontown, Ala., USA, a high caries risk community (i.

Reporting stAndards for research in PedIatric Dentistry (RAPID): an expert consensus-based statement.

Background: Reporting guidelines for different study designs are currently available to report studies with accuracy and transparency. There is a need to develop supplementary guideline items that are specific to areas within Pediatric Dentistry. This study aims to develop Reporting stAndards for research in PedIatric Dentistry (RAPID) guidelines using a pre-defined expert consensus-based Delphi process.

Children's detailed non-water beverage consumption habits and longitudinal early childhood caries experiences.

Objectives: Sugar-sweetened beverages and dental caries are prevalent among children. Measurement choices could explain their inconsistent relationships. This study evaluated associations between detailed baseline non-water beverage consumption (NWBC) behaviors and longitudinal early childhood caries (ECC) experiences in young, high-caries risk children.

Overestimation of Early Childhood Caries Using the dmfs Index.

The preferred epidemiological caries assessment method is the decayed, missing, and filled surfaces (dmfs) score, which records all crowned/missing primary teeth's surfaces as carious. The purpose of this study was to evaluate the dmfs score's accuracy in capturing caries-affected (versus treated) surfaces of crowned/extracted teeth. A high-caries risk cohort of children, eight to 18 months old at baseline, were recruited from a nonfluoridated, rural, minority, and low-income community.

Models to Predict Future Permanent Tooth Caries Incidence in Children Using Primary Teeth Caries Experience.

To assess permanent tooth caries incidence (ΔDMFS) among a cohort of African American children using the presence of any caries experience(decayed, missing, and filled surfaces-dmfs) in primary teeth and the presence of untreated primary tooth caries (ds) in two separate models. Data from a prospective study was used to apply two models with different clinical for predicting DMFS from ages six through 12 years. The first model used dmfs, and the second model used ds as predictors (both at age six years).

Average area under the curve: An alternative method for quantifying the dental caries experience in longitudinal studies.

Objectives: Field-traditional decayed, missing, filled surfaces (dmfs) increments require complete follow-up, only using initial and final visits. Repeated dmfs scores complicate sophisticated statistical models, limiting their utility. Elsewhere, area under the curve (AUC) uses all repeated measures to summarize data.

Streptococcus mutans serotypes and collagen-binding proteins Cnm/Cbm in children with caries analysed by PCR.

Streptococcus mutans, a primary bacterium associated with dental caries, has four known clinical serotypes (c, e, fand k). Certain serotypes, the presence of multiple serotypes and strains with collagen-binding proteins (CBP, Cnm and Cbm) have been linked with systemic disease. Evaluation of S mutans serotype distribution and caries association is needed in the United States.

Survival analysis of caries incidence in African-American school-aged children.

Objectives: To conduct an assessment of time-dependent covariates related to dental caries of the permanent dentition among a low socioeconomic status, understudied cohort of children, incorporating time-dependent covariates through the application of extended Cox proportional hazards modeling.

Methods: This study modeled the time to first cavitated dental caries in permanent teeth among school-aged children and assessed factors associated with this event. A cohort of 98 low socioeconomic status African-American children with mean age of 5.

Surgical Management and Evaluation of the Craniofacial Growth and Morphology in Cleidocranial Dysplasia.

Cleidocranial dysplasia (CCD, MIM 119600) is a rare autosomal dominant disorder affecting bone, cartilage, craniofacial growth, and tooth formation leading to supernumerary teeth. Few reports delineate the genotype-phenotype correlations related to the variations in craniofacial morphology and patterning of the dentition and the complexity of treating patient's malocclusion. Successful management of the craniofacial deformities in patients with CCD requires a multidisciplinary team of healthcare specialists.

Transmission patterns of Streptococcus mutans demonstrated by a combined rep-PCR and MLST approach.

Objective: Clinical typing methods of the oral pathogen Streptococcus mutans with molecular analysis can be very specific, but expensive. Repetitive extragenic palindromic PCR (rep-PCR) is a relatively inexpensive pre-screening alternative for isolate selection for additional analyses. This study evaluated the prediction accuracy of using rep-PCR to identify S.

Mutans Streptococci and Dental Caries: A New Statistical Modeling Approach.

Survival analyses have been used to overcome some of the limitations encountered with other statistical analyses. Although extended Cox hazard modeling with time-dependent variables has been utilized in several medical studies, it has never been utilized in assessing the complex relationship between mutans streptococci (MS) acquisition (time-dependent covariate) and time to having dental caries (outcome). This study involved secondary analyses of data from a prospective study conducted at the University of Alabama at Birmingham.

Potential Risk for Localized Aggressive Periodontitis in African American Preadolescent Children.

Purpose: This study aimed to evaluate the potential risk for localized aggressive periodontitis (LAgP) in African American children by detection of the potential periodontal pathogen Aggregatibacter actinomycetemcomitans (Aa) using polymerase chain reaction (PCR) and microbiome analysis.

Methods: Twenty-one pre-adolescents (age range equals 10.7 to 13.

Association Between Early Childhood Caries and Colonization with Genotypes From Mothers.

Purpose: The purpose of this study was to evaluate Streptococcus mutans genotypes (GT) between mother and child (M-C) in a high caries risk cohort to explore the association with early childhood caries (ECC).

Methods: Sixty-nine infants (each approximately one year old) had periodic oral examinations (dmfs) and microbial samples collected from dental plaque, saliva, and other oral surfaces. Their mothers had an examination and plaque collected.

Genetic Diversity and Evidence for Transmission of Streptococcus mutans by DiversiLab rep-PCR.

This two-part study investigated the genetic diversity and transmission of Streptococcus mutans using the DiversiLab repetitive extragenic palindromic PCR (rep-PCR) approach. For children with S. mutans and participating household members, analysis for evidence of unrelated child-to-child as well as intra-familial transmission was evaluated based on commonality of genotypes.

Dental Caries in High-risk, School-age African American Children in Alabama: A Six-year Prospective.

Purpose: The purpose of this study was to assess the prevalence and incidence of dental caries in school-age African American children who received semi-annual fluoride varnish applications.

Methods: A cohort of six-year-old high caries-risk African American children (n equals 98) was recruited in Uniontown, Ala., USA, and followed for six years.

Assessment of clonality and serotypes of Streptococcus mutans among children by multilocus sequence typing.

Studies using multilocus sequence typing (MLST) have demonstrated that Streptococcus mutans isolates are genetically diverse. Our laboratory previously demonstrated clonality of S. mutans using MLST but could not discount the possibility of sampling bias.

Assessment of two multilocus sequence typing (MLST) schemes available for Streptococcus mutans.

Objective: Two multilocus sequencing typing (MLST) schemes are currently available for Streptococcus mutans. The first, introduced by Nakano et al. in 2007, consists of 8 conserved housekeeping genes.

Evaluation of ciprofloxacin and metronidazole encapsulated biomimetic nanomatrix gel on and .

Background: A triple antibiotic mixture (ciprofloxacin; CF, metronidazole; MN, and minocycline; MC) has been used for dental root canal medicaments in pulp regeneration therapy. However, tooth discolorations, cervical root fractures, and inadequate pulp-dentin formation have been reported due to the triple antibiotic regimen. Therefore, an antibiotic encapsulated biomimetic nanomatrix gel was developed to minimize the clinical limitations and maximize a natural healing process in root canal infections.

Factors associated with early childhood caries incidence among high caries-risk children.

Objectives: To assess the relationships between different behavioral factors and Early Childhood Caries (ECC) in African-American pre-school children.

Methods: Ninety-six African-American children aged 3-22 months old at baseline were recruited from a high caries risk, non-fluoridated African-American community in Uniontown, Alabama. The children had dental examinations annually following World Health Organization (WHO) criteria at mean ages 1.