Publications by authors named "Nobuo Miyazaki"
Article Synopsis
- * An alpaca was immunized with IgG fragments, and its antibody development was traced over months; researchers generated antibodies from the most abundant clusters found in sequences, discovering significant mutations in antigen-responsive clusters that showed continuous turnover.
- * The study achieved over 80% success in predicting clusters of antibodies reacting to the antigen without prior empirical screening and confirmed the method's applicability in other contexts, demonstrating its ability to identify rare immune-responsive sequences.
Article Synopsis
- VHH antibodies, derived from camelids, offer benefits like small size, easy production in bacteria, and adaptability for improvements.
- The study created a VHH phage display library from alpaca B cells immunized with Legionella pneumophila to develop a detection system.
- A sensitive latex agglutination assay for detecting Legionella was successfully established, aiming to improve detection of this serious pneumonia-causing pathogen.
Kudoa septempunctata is the causative agent of a food-borne disease associated with the ingestion of raw olive flounder. As the current qRT-PCR method for its detection is time-consuming, a rapid and simple method is required. Recently, a new real-time loop-mediated isothermal amplification (LAMP) method and an immunochromatography method, whose sensitivities are intended to be compatible with that designated in the official analytical method (10(5) spores/g olive flounder), have been developed.
View Article and Find Full Text PDFThe antigen-binding domain of camelid dimeric heavy chain antibodies, known as VHH or Nanobody, has much potential in pharmaceutical and industrial applications. To establish the isolation process of antigen-specific VHH, a VHH phage library was constructed with a diversity of 8.4 × 10(7) from cDNA of peripheral blood mononuclear cells of an alpaca (Lama pacos) immunized with a fragment of IZUMO1 (IZUMO1PFF) as a model antigen.
View Article and Find Full Text PDFAnti-IZUMO1PFF VHH (variable domain of camelid heavy chain antibody) clones, N6 and N15, from immunized alpaca (Lama pacos) phage library were efficiently expressed and their VHH products were secreted into the culture medium of Brevibacillus choshinensis HPD31-SP3, e.g., at a level of 26-95mg in 100ml conventional flask culture.
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