-Adenosylhomocysteine Analogue of a Fairy Chemical, Imidazole-4-carboxamide, as its Metabolite in Rice and Yeast and Synthetic Investigations of Related Compounds.

During the course of our investigations of fairy chemicals (FCs), we found -ICAr-H (), as a metabolite of imidazole-4-carboxamide (ICA) in rice and yeast (). In order to determine its absolute configuration, an efficient synthetic method of was developed. This synthetic strategy was applicable to the preparation of analogues of that might be biologically very important, such as -ICAr-M (), -AICAr-H (), and -AICAr-M ().

Characterization of Colibactin-Producing Escherichia coli Isolated from Japanese Patients with Colorectal Cancer.

We investigated the relationship between colibactin-producing (clb) Escherichia coli and colorectal adenocarcinoma. In total, 729 E. coli colonies were isolated from tumor and surrounding non-tumor regions in resected specimens from 34 Japanese patients; 450 colonies were from the tumor regions and 279 from the non-tumor regions.

Ribosides and Ribotide of a Fairy Chemical, Imidazole-4-carboxamide, as Its Metabolites in Rice.

The metabolism of imidazole-4-carboxamide (ICA) in plants has been unknown. Two metabolites ( and ) were isolated from ICA-treated rice, and their structures were determined by spectroscopic analysis including the single-crystal X-ray diffraction technique and synthesis. The ribotide of ICA (), whose existence was predicted, was also synthesized and detected from the treated rice by LC-MS/MS.

A Fairy Chemical, Imidazole-4-carboxamide, is Produced on a Novel Purine Metabolic Pathway in Rice.

Rings or arcs of fungus-regulated plant growth occurring on the floor of woodlands and grasslands are commonly called "fairy rings". Fairy chemicals, 2-azahypoxanthine (AHX), imidazole-4-carboxamide (ICA), and 2-aza-8-oxohypoxanthine (AOH), are plant growth regulators involved in the phenomenon. The endogeny and biosynthetic pathways of AHX and AOH in plants have already been proven, however, those of ICA have remained unclear.

Activity-Based Probe for Screening of High-Colibactin Producers from Clinical Samples.

While high-colibactin-producing Escherichia coli is thought to be associated with colorectal oncogenesis, this study is complicated part due to an inability to isolate colibactin adequately. Here, we created fluorescent probes activated by ClbP, the colibactin-maturing peptidase, to identify high-colibactin-producing strains. Our probe served as a valuable clinical diagnostic tool that allowed simple high-throughput diagnostic screening of clinical samples.