Accurate nano-injection system for capillary gas chromatography.

The first version of nano-injection device for capillary gas chromatography (cGC) based on inkjet microchip was developed. The nano-injector could accurately control the injection volume in nano-liter, even pico-liter range. Its configuration and mechanism were discussed in detail.

Preparation of two-dimensionally ordered microbeads structure dispensed with an ink-jet and its application to ELISA.

In order to the increase sensitivity of enzyme-linked immunosorbent assay (ELISA) using micro-droplet reaction system formed by ink-jet, we employed small dots of two-dimensionally well-ordered structure of polystyrene (PS) microbeads (710 +/- 25 microm in diameter) formed on the surface of polydimethyl siloxane (PDMS). An aqueous suspension of polystyrene microbeads (5 microm) was ejected on the PDMS plate with ink-jet. The PS beads were automatically assembled by capillary force accompanied with solvent evaporation.

Development of an ultra-micro sample injector for gas chromatography using an ink-jet microchip.

An ultra-micro sample injector for gas chromatography (GC) was developed. An ink-jet microchip, originally used for industrial recorder, was modified at the edge near to an orifice, and fixed into the GC. In order to evaluate the characteristics of this injector, a sample injector and a thermal conductive detector (TCD) were connected directly, while water was used as the test sample.

Development of a surface-reaction system in a nanoliter droplet made by an ink-jet microchip.

A surface-reaction system in a nanoliter water pool using an ink-jet microchip was developed. The reaction system in the nanodroplets formed on a poly(dimethylsiloxane) (PDMS) coated glass slide increased the diffusion-controlled reaction without using a nano-pump, specialized connector or highly sensitive detector. When nanoliter droplets were placed on the PDMS surface with a distance of 100 microm between them by the ink-jet microchip, the repeatabilities of the fluorescence intensity were 2.