Development of a Gene and Nucleic Acid Delivery System for Skeletal Muscle Administration via Limb Perfusion Using Nanobubbles and Ultrasound.

Strategies for gene and nucleic acid delivery to skeletal muscles have been extensively explored to treat Duchenne muscular dystrophy (DMD) and other neuromuscular diseases. Of these, effective intravascular delivery of naked plasmid DNA (pDNA) and nucleic acids into muscles is an attractive approach, given the high capillary density in close contact with myofibers. We developed lipid-based nanobubbles (NBs) using polyethylene-glycol-modified liposomes and an echo-contrast gas and found that these NBs could improve tissue permeability by ultrasound (US)-induced cavitation.

Sonoluminescence from ultra-high temperature and pressure cavitation produced by a narrow water jet.

This work developed a small-scale processing apparatus for ultra-high temperature and ultra-high-pressure cavitation (UTPC) incorporating a small diameter (0.1 mm) water jet nozzle. This instrumentation comprised a swirl flow nozzle (SFN) installed on the water jet nozzle so as to obtain UTPC from a multifunction cavitation (MFC) setup.