Publications by authors named "Nobuaki Arita"
Sphingoid long-chain bases are essential intermediates of ceramides and complex sphingolipids, and function in the regulation of various signal transduction systems. Previously, we found that, in budding yeast, intracellularly accumulated dihydrosphingosine (DHS) causes mitochondrial reactive-oxygen species (ROS)-mediated cytotoxicity, which is much stronger than phytosphingosine. In this study, we screened for suppressor mutations that confer resistance to DHS, and identified RTG2, which encodes upregulation of the mitochondrial retrograde signaling pathway (RTG pathway).
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- In eukaryotic cells, sphingoid long-chain bases (LCBs) are present at lower levels compared to complex sphingolipids and ceramides, and their balance is crucial for cell function.
- In Saccharomyces cerevisiae, adding phytosphingosine (PHS) severely affects tryptophan-auxotrophic cells, while dihydrosphingosine (DHS) is initially less harmful, but shows greater toxicity in tryptophan-prototrophic cells once metabolized.
- The cytotoxic effects of DHS are linked to increased reactive oxygen species from mitochondria, which can be influenced by mitochondrial DNA status and antioxidant levels; thus, DHS is more cytotoxic than