The Management of Iatrogenic Ureterovaginal Fistula in a Resource-Limited Setting: A 12-Year Experience at Four Fistula Surgery Centers in Uganda.

Background Ureterovaginal fistulae usually follow iatrogenic injury to the ureter during pelvic surgery. This manifests as urine incontinence and results in serious psychosocial effects on women. Ureterovaginal fistulae unlike vesicovaginal fistulae present challenges in diagnosis and management especially in resource-constrained settings.

Best practices for acceptability of GM crops field trials conclusions: lessons for Africa.

The ability to transfer information about the performance, safety, and environmental impacts of a genetically modified (GM) crop from confined field trials (CFTs) conducted in one location to another is increasingly gaining importance in biosafety regulatory assessment and decision-making. The CFT process can be expensive, time-consuming, and logistically challenging. Data transportability can help overcome these challenges by allowing the use of data obtained from CFTs conducted in one country to inform regulatory decision-making in another country.

Risk factors for recurrence of pelvic organ prolapse after vaginal surgery among Ugandan women: a prospective cohort study.

Introduction And Hypothesis: This study was aimed at determining the recurrence rate and risk factors for the recurrence of pelvic organ prolapse (POP), at 1 year post-vaginal reconstructive surgery in a resource-limited setting.

Methods: We enrolled women who underwent vaginal surgery for POP at the urogynecology unit of Mbarara Regional Referral Hospital (MRRH) in southwestern Uganda between December 2018 and February 2020. The surgeries that were performed include anterior colporrhaphy for cystocele, posterior colporrhaphy for rectocele, vaginal hysterectomy with vault suspension for uterine prolapse, and cervicopexy in those with uterine prolapse where uterine-sparing surgery was desired.

Preventive malaria treatment among school-aged children in sub-Saharan Africa: a systematic review and meta-analyses.

Background: The burden of malaria infection in sub-Saharan Africa among school-aged children aged 5-15 years is underappreciated and represents an important source of human-to-mosquito transmission of Plasmodium falciparum. Additional interventions are needed to control and eliminate malaria. We aimed to assess whether preventive treatment of malaria might be an effective means of reducing P falciparum infection and anaemia in school-aged children and lowering parasite transmission.

Effects of DCM Leaf Extract of (Fresen) on Locomotor Activity, Anxiety, and Exploration-Like Behaviors in High-Fat Diet-Induced Obese Rats.

Obesity is the main component of metabolic syndromes involving distinct etiologies that target different underlying behavioral and physiological functions within the brain structures and neuronal circuits. An alteration in the neuronal circuitry stemming from abdominal or central obesity stimulates a cascade of changes in neurochemical signaling that directly or indirectly mediate spontaneously emitted behaviors such as locomotor activity patterns, anxiety, and exploration. Pharmacological agents available for the treatment of neurologic disorders have been associated with limited potency and intolerable adverse effects.

Regional legal and policy instruments for addressing LGBT exclusion in Africa.

The vulnerability of lesbian, gay, bisexual and transgender (LGBT) persons in Africa to public health and other risks is heightened by their exclusion from socio-economic opportunities and services. We analysed existing regional-level legal and policy instruments and treaties for the opportunities they offer to tackle the exclusion of LGBT persons in Africa. We identified seven key living legal and policy instruments, formulated and adopted between 1981 and 2018, by the African Union (AU) or its precursor, the Organization of African Unity.

Anti-obesity effects of dichloromethane leaf extract of in high fat diet-induced obese rats.

Background: Obesity is a chronic metabolic disorder characterized by increased adipose tissue mass due to positive energy balance. Prescription of anti-obesity drugs can be useful adjuncts to diet and exercise for obese patients who have failed to achieve weight loss. However, these drugs are ineffective and are associated with adverse effects.

In Vitro Antioxidant Properties of Dichloromethanolic Leaf Extract of (Fresen) as a Promising Antiobesity Drug.

The acquisition of ethnobotanical information from traditional practitioners remains an empirical aspect of understanding the ethnopharmacology research. However, integration of information on chemical composition of plant extracts and their pharmacological activities forms a key resource for synthesis of new and effective therapeutics. In traditional African medicine, has folkloric remedies against obesity and its associated oxidative stress-mediated complications.

Modulation of Cognition: The Role of on Spatial Learning and Memory Retention in High-Fat Diet-Induced Obese Rats.

Chronic exposures to high-fat diets are linked to neuropathological changes that culminate in obesity-related cognitive dysfunction and brain alteration. Learning, memory performance, and executive function are the main domains affected by an obesogenic diet. There are limited effective therapies for addressing cognitive deficits.

A modified neo-vagina procedure in a low resource urogynecological unit: a case report of a 21 year old with Mayer-Rokitansky-Küster-Hauser (mrkh) Syndrome operated at Mbarara referral hospital, Southwestern Uganda.

Background: Although vaginal agenesis as may occur in Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome is a rare condition, it is associated with not only anatomical problems but also serious psychological and social problems like painful sexual intercourse, primary amenorrhea and infertility. Surgery, which is aimed at reconstruction of a vagina of adequate length and width to serve the function, is the main method of treatment. Many methods for vaginal reconstruction have been described but each has its complications and limitations.

Deposition of hermetic silver shells onto copper flakes.

Continuous silver shells were deposited on copper flakes using a two-stage precipitation process. A tightly packed layer of silver nanoparticles was first formed on the surface of the base metal by galvanic displacement. The size of the noble metal particles and their distribution on the substrate were controlled using complexing agents and dispersants.

Nitrilotriacetic acid: a novel reducing agent for synthesizing colloidal gold.

We report for the first time that nitrilotriacetic acid (NTA) is an effective reductant for the preparation of stable dispersions of uniform gold nanoparticles. The method described is capable of generating stable sols with a metal concentration as high as 1.5×10(-3)moldm(-3).

Deposition of continuous nickel shells on polymer microspheres.

Anisotropic conductive adhesives (ACAs) are widely used as interconnect materials in the manufacturing of LCD screens. To be integrated in a broader range of applications, several technical and economical issues still need to be addressed. Encapsulating the polymer particles within continuous, compact, and adhering metallic conductive shells is one of these challenges.

Deposition of continuous platinum shells on gold nanoparticles by chemical precipitation.

Continuous platinum shells consisting of ~5 atomic layers were deposited onto preformed gold seeds in aqueous medium by reducing hexachloroplatinic acid with ascorbic acid. By controlling the reduction kinetics of Pt(IV) species and the properties of the substrate, it was possible to ensure a slow and controlled deposition of platinum atoms onto the gold cores. Electrochemical evaluations revealed the presence of a compact platinum shell.

Plasmodium falciparum transmission and aridity: a Kenyan experience from the dry lands of Baringo and its implications for Anopheles arabiensis control.

Background: The ecology of malaria vectors particularly in semi-arid areas of Africa is poorly understood. Accurate knowledge on this subject will boost current efforts to reduce the burden of malaria in sub-Saharan Africa. The objective of this study was to describe the dynamics of malaria transmission in two model semi-arid sites (Kamarimar and Tirion) in Baringo in Kenya.

Dry season ecology of Anopheles gambiae complex mosquitoes at larval habitats in two traditionally semi-arid villages in Baringo, Kenya.

Background: Pre-adult stages of malaria vectors in semi-arid areas are confronted with highly variable and challenging climatic conditions. The objective of this study was to determine which larval habitat types are most productive in terms of larval densities in the dry and wet seasons within semi-arid environments, and how vector species productivity is partitioned over time.

Methods: Larval habitats were mapped and larvae sampled longitudinally using standard dipping techniques.

Electrochemical quantification of serotonin in the live embryonic zebrafish intestine.

We monitored real-time in vivo levels of serotonin release in the digestive system of intact zebrafish embryos during early development (5 days postfertilization, dpf) using differential pulse voltammetry with implanted carbon fiber microelectrodes modified with carbon nanotubes dispersed in nafion. A detection limit of 1 nM, a linear range between 5 and 200 nM, and a sensitivity of 83.65 nA x microM(-1) were recorded.

Amperometric detection of dopamine in vivo with an enzyme based carbon fiber microbiosensor.

We developed a novel implantable enzyme-based carbon fiber biosensor for in vivo monitoring of dopamine. The biosensor is fabricated using tyrosinase immobilized in a biocompatible matrix consisting of a biopolymer, chitosan and ceria-based metal oxides, deposited onto the surface of a carbon fiber microelectrode with a diameter of approximately 100 microm. Tyrosinase catalyzes the conversion of dopamine to o-dopaquinone, and the reduction of o-dopaquinone, which requires a low potential difference, was detected electrochemically.

Detection and prediction of concentrations of neurotransmitters using voltammetry and pattern recognition.

Neurotransmitters (NTs) are substances in the brain which are responsible for the transmission of neurological impulses. Changes in their concentrations are associated with numerous behavioral and physiological processes and neurological disorders. As opposed to the traditional chromatographic and capillary electrophoresis, using electrochemical sensors is a fast and inexpensive way to determine concentrations of NTs.

JEM Spotlight: Applications of advanced nanomaterials for environmental monitoring.

Rapid progress of the nanotechnology and advanced nanomaterials production offers significant opportunities for a wide range of applications for detection and remediation of a broad range of environmental contaminants. The convergence of analytical techniques and nanotechnology provides attractive possibilities for development of miniaturized, rapid, ultrasensitive and inexpensive methods for in situ and field-based environmental monitoring devices. This review provides an overview of the various nanoparticles and nanostructures used for this purpose, their integration into functional analytical devices, applications as electrode materials and gas sensing nanoprobes, in biosensors and as capture probes in immunomagnetic separations.

Costs and cost-effectiveness of delivering intermittent preventive treatment through schools in western Kenya.

Background: Awareness of the potential impact of malaria among school-age children has stimulated investigation into malaria interventions that can be delivered through schools. However, little evidence is available on the costs and cost-effectiveness of intervention options. This paper evaluates the costs and cost-effectiveness of intermittent preventive treatment (IPT) as delivered by teachers in schools in western Kenya.

Mixed ceria-based metal oxides biosensor for operation in oxygen restrictive environments.

The unique catalytic, electrochemical, and oxygen storage properties of ceria and mixed ceria/titania hybrid composites were used to fabricate a new type of electrochemical enzyme biosensor. These materials provided increased analytical performance and possibilities for operation in oxygen-free conditions of an oxidase enzyme biosensor using tyrosinase as a model example. The investigation of the enzymatic reaction in the presence and absence of oxygen was first carried out using cyclic voltammetry.

Relationships between anaemia and parasitic infections in Kenyan schoolchildren: a Bayesian hierarchical modelling approach.

Anaemia is multi-factorial in origin and disentangling its aetiology remains problematic, with surprisingly few studies investigating the relative contribution of different parasitic infections to anaemia amongst schoolchildren. We report cross-sectional data on haemoglobin, malaria parasitaemia, helminth infection and undernutrition among 1523 schoolchildren enrolled in classes 5 and 6 (aged 10-21 years) in 30 primary schools in western Kenya. Bayesian hierarchical modelling was used to investigate putative relationships.