Publications by authors named "Nisha Mukund"
Ureibacillus thermophilus strain LM102 is a facultative thermophile with growth in range 37 °C-60 °C. Upon identification using the 16S rRNA marker, it showed highest similarity of 99.8% with U.
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- The study addresses the issues of fossil fuel shortages and environmental pollution by exploring the use of ethanol production from cellulosic materials, specifically using the bacteria Clostridium thermocellum.
- Researchers genetically modified C. thermocellum by introducing pyruvate carboxylase and alcohol dehydrogenase genes from Zymomonas mobilis, creating different recombinant strains that could enhance ethanol production.
- Results showed that the modified strain with the pyruvate decarboxylase gene resulted in a two-fold increase in ethanol production compared to the wild type, suggesting potential for further improvements in ethanol yield through strain optimization.
Pyruvate ferredoxin oxidoreductase is the crucial enzyme that involves in bioethanol synthesis pathway of Clostridium thermocellum. It is an ethanologenic organism but has been investigated less on its enzyme structure. The amino acid sequence of Pyruvate ferredoxin oxidoreductase was derived from UNIPROT and the screened crystal structure was taken as the template for homology modeling using MODELLER 9V11.
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